Table 1

Synthetic oligonucleotide primers used for amplification and sequencing of TMPRSS3 exons

Exon	Primer sequence (F) (5′→3′)	Primer sequence (R) (5′→3′)	Product size (bp)	Annealing temperature (°C)	Buffer^1-150
1	CTCTGGTCTCCTTGGCATTGTG	CTCTCAAAGCCCTTTCCATTGC	387	60/50^1-151
2	GATGCACCTGATGCTACAAG	GGACAGTCAGTCACATTGGTC	286	55	12
3	GGACTGAACTAGAGAATGTGCC	GACAAAGCCATGAGCATGGC	510	55
4	AGGGGGACAGTTGTTAGTGTTGC	TACAGATGGGAAGGGTCAGGGTTG	261	60	12
5	TGTGGAGAAACCCCTGCCTATG	GATGTGAGGATGTAATCTGAGAGCG	323	55	9
6	GACTCGCACATCGGTTGAATG	ATACTCCCTCAGGTTCTCACACCC	387	55	12
7	GTGTGACCTCATCCTCATGG	CTCTGAGGGCAAGGAGATAG	293	60	9
8	TAGAGCTGCTGTGAGCTCTG	AGACTCCTCTCCAACTGTAC	438	55	10
9	GGACCACATCTTGCCTGATAACC	AAAGCACACAGCCCACGAAG	694	60	12
10	CTCCTGCTGTGAGCTGATCG	CGAGCAGCTGACATGCACTC	393	55	10
11	GTCTCAGCATGCGCCTTCTG	CCCACGCAGAGCCAGATCAC	407	60
12	TGGGTCATCCATTGGGACATC	TCTCTGTTTTCAGCACAAGCGTC	460	55	12
13	TACGGAAGTGACGGAACTGTCG	CTTGAAGGTTGTGCTGGAATCAG	442	60

↵1-150 The number of Opti-Prime buffer (Stratagene) used for PCR amplification. Other reactions were performed with a standard PCR buffer (PE Applied Biosystems), with 1.5 mmol/l MgCl₂.
↵1-151 Touchdown.