Amplification conditions, primers and sizes of PCR products for KCNE1 (a) and KCNE2 (b) coding region. PCR programmes (a-g) are given in Methods. The value following programme (a) indicates the annealing temperature for each PCR amplicon. Primers for KCNE1 and KCNE2 have been reported previously16 19
| A Exon | Size (bp) | Primers | PCR fragment (bp) | PCR programme | Special conditions | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| 3 | 437+ | F: CTGCAGCAGTGGAACCTTAATG | 264 | (f) | ||||||
| R: GTTCGAGTGCTCCAGCTTCTTG | ||||||||||
| F: GGGCATCATGCTGAGCTACAT | 231 | (f) | ||||||||
| R: TTAGCCAGTGGTGGGGTTCA | ||||||||||
|
B Coding region (single exon) | Size (bp) | Primers |
PCR fragment (bp) |
PCR programme |
Special conditions | |||||
| 371 | F: CCGTTTTCCTAACCTTGTTCG | 291 | (a) 55°C | |||||||
| R: GTTCCCGTCTCTTGGATTTCA | ||||||||||
| F: AATGTTCTCTTTCATCATCGTG | 239 | (a) 55°C | ||||||||
| R: TGTCTGGACGTCAGATGTTAG | ||||||||||