Locus/region3-150 (distance from 10ptel) | Methods3-151 (FISH probe) | Family A | Family B | Family C | Family D | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
F | P | M | F | P | M | F | P | M | F | P | M | ||||||
10p telomere region | FISH (2189b6/cosmid) | – | – | – | + | ||||||||||||
D10S552 (14.33 cM) | FISH (809F09/YAC) | – | – | – | + | ||||||||||||
D10S552 | MS | 5 | 43-152 | 4 | 1 | 13-152 | 3, 4 | 1, 4 | 53-152 | 3, 5 | 1, 4 | 1 | 1, 2 | ||||
D10S189 (19.00 cM) | MS | 1, 3 | 3 | 1, 3 | 3, 4 | 3 | 3, 4 | 1, 4 | 1 | 1, 4 | 2, 3 | 1, 2§ | 1, 3 | ||||
D10S1751 (20.75 cM) | MS | 1, 3 | 1 | 1, 2 | 3, 5 | 53-152 | 2 | 1, 4 | 53-152 | 2, 5 | 1, 2 | 2 | 2 | ||||
GATA3 | FISH (554F11/BAC) | – | – | – | – | ||||||||||||
D10S1779 (22.78 cM) | FISH (42I17/BAC) | – | – | – | – | ||||||||||||
D10S1779 | MS | 1, 2 | 2 | 1, 2 | 3 | 3 | 3, 4 | 1, 2 | 2 | 1, 2 | 1, 3 | 3 | 3 | ||||
D10S226 (27.19 cM) | FISH (182F10/BAC) | – | – | – | – | ||||||||||||
D10S226 | MS | 2, 4 | 2 | 2, 5 | 1, 2 | 1 | 1, 5 | 4, 5 | 5 | 3, 5 | 1, 4 | 53-152 | 2, 5 | ||||
D10S1720 (27.19 cM) | FISH (249K20/PAC) | – | – | – | – | ||||||||||||
D10S1720 | MS | 2, 6 | 2 | 2, 3 | 1, 4 | 1 | 1 | 2, 4 | 33-152 | 2, 3 | 3, 5 | 3 | 1, 3 | ||||
D10S1649 (27.19 cM) | MS | 2, 4 | 3, 4§ | 3, 6 | 1, 6 | 3, 6§ | 3, 5 | 4, 6 | 73-152 | 3, 7 | 2, 3 | 13-152 | 1, 4 | ||||
D10S465 (28.31 cM) | MS | 2, 3 | 2, 3§ | 2, 3 | 2 | 2, 3§ | 3 | 1, 3 | 23-152 | 2, 3 | 2 | 33-152 | 3 | ||||
D10S547 (29.15 cM) | MS | 1, 2 | 2 | 2 | 2 | 2 | 2 | 2, 4 | 33-152 | 2, 3 | 2, 3 | 2, 3§ | 2, 3 | ||||
D10S585 (30.00 cM) | FISH (765A04/YAC) | + | + | + | + | ||||||||||||
D10S585 | MS | 1 | 1, 3§ | 3, 4 | 5 | 4, 5§ | 4, 6 | 2 | 2, 6§ | 1, 6 | 4, 6 | 4, 6§ | 6 | ||||
D10S223 (32.80 cM) | MS | 1 | 1 | 1, 2 | 1, 2 | 1, 2§ | 1, 2 | 1 | 1 | 1 | 2, 3 | 1, 3§ | 1 | ||||
D10S191 (37.90 cM) | MS | 2, 3 | 2, 7§ | 3, 7 | 1, 2 | 1, 2§ | 1 | 2, 5 | 2, 4§ | 2, 4 | 2, 6 | 3, 6§ | 3, 7 |
F, father; P, proband; M, mother; FISH, fluorescence in situ hybridisation analysis; MS, microsatellite analysis.
↵3-150 The locus order and the genetic distance from the 10p telomere are primarily based on the chromosome 10 linkage map of the Marshfield Clinic (http://research.marshfieldclinic.org/genetics/).
↵3-151 Resources for FISH probes: Ning et al,16 Children's Hospital, Oakland Research Institute (http://www.chori.org/bacpac/), Incyte Genomics (http://www.incyte.com/reagents/index.shtml), and Foundation Jean Dausset CEPH (http://www.cephb.fr/).
Resources for MS primers: Genome Database (http://www.gdb.org/).
The (+) and (–) symbols for FISH analysis represent the presence or absence of the examined loci/region on the abnormal chromosome 10, respectively.
The alleles determined by MS analysis are arbitrarily indicated by Arabic numbers according to their size:
↵3-152 confirmed hemizygosity and§confirmed heterozygosity.