Table 1

Primers and PCR conditions used for the MECP2 coding sequence screening

Exons		Primers	Annealing T (°C)	PCR product (bp)
1				F : ATTTTCTTTGTTTTAGGCTCCA	50		185
1				R : GGCCAAACCAGGACATATAC	50		185
2		A		F : GGCATGTTCTCTGTGATACT	58		182
		A		R : TGCCTCTGCGGGCTCAGCA	58		182
		B		F : AAGAGAAAGAGGGCAAGCAT	58		296
		B		R : CTGTAGAGATAGGAGTTGCT	58		296
3		A		F : TGTGTCTTTCTGTTTGTCCC	58		181
		A		R : GATTTGGGCTTCTTAGGTGG	58		181
		B		F : CCTCCCGGCGAGAGCAGAAA	64		243
		B		R : TGACCTGGGTGGATGTGGTG	64		243
		C		F : TGCCTTTTCAAACTTCGCCAG	58		280
		C		R : TTGACCTCGATGCTGACCGT	58		280
		D		F : GATCTGTGCAGGAGACCGTA	62		250
		D		R : TTTGGGGACTCTGAGTGGTG	62		250
		E		F : GCAGCAGCAGCGCCTCCTCA	68		264
		E		R : TGGCAACCGCGGGCTGAGTC	68		264
		F		F : TGCCCCAAGGAGCCAGCTAA	64		202
		F		R : GCTTTGCAATCCGCTCCGTG	64		202