Primers and PCR conditions used for the MECP2 coding sequence screening
Exons | Primers | Annealing T (°C) | PCR product (bp) | |||||
---|---|---|---|---|---|---|---|---|
1 | F : ATTTTCTTTGTTTTAGGCTCCA | 50 | 185 | |||||
R : GGCCAAACCAGGACATATAC | ||||||||
2 | A | F : GGCATGTTCTCTGTGATACT | 58 | 182 | ||||
R : TGCCTCTGCGGGCTCAGCA | ||||||||
B | F : AAGAGAAAGAGGGCAAGCAT | 58 | 296 | |||||
R : CTGTAGAGATAGGAGTTGCT | ||||||||
3 | A | F : TGTGTCTTTCTGTTTGTCCC | 58 | 181 | ||||
R : GATTTGGGCTTCTTAGGTGG | ||||||||
B | F : CCTCCCGGCGAGAGCAGAAA | 64 | 243 | |||||
R : TGACCTGGGTGGATGTGGTG | ||||||||
C | F : TGCCTTTTCAAACTTCGCCAG | 58 | 280 | |||||
R : TTGACCTCGATGCTGACCGT | ||||||||
D | F : GATCTGTGCAGGAGACCGTA | 62 | 250 | |||||
R : TTTGGGGACTCTGAGTGGTG | ||||||||
E | F : GCAGCAGCAGCGCCTCCTCA | 68 | 264 | |||||
R : TGGCAACCGCGGGCTGAGTC | ||||||||
F | F : TGCCCCAAGGAGCCAGCTAA | 64 | 202 | |||||
R : GCTTTGCAATCCGCTCCGTG |