Primers for PCR amplification of the NPC1 gene exons
| Name | Sequence | Localisation1-150 | PCR products (bp) | ||
| ex1 | 1FW | 5′-CTG AAA CAG CCC GGG GAA GTA G-3′ | −87 | 232 | |
| 57RV | 5′-GCC TGA GCC GTC GCT GGG CC-3′ | +88 | |||
| ex2 | 58FW | 5′-ACC ATT GAG ACC CTG GTA AC-3′ | −47 | 207 | |
| 180RV | 5′-CAT TTT GTG TTC CCA GTG CC-3′ | +37 | |||
| ex3 | 181FW | 5′-GAC CTT ACT CTA ACT GTT GCC-3′ | −53 | 230 | |
| 287RV | 5′-CAC AAG TAT CTA CAG CCC AG-3′ | +43 | |||
| ex4 | 288FW | 5′-CTT GCT GGC CCT ATT ATG TGT G-3′ | −56 | 292 | |
| 463RV | 5′-CAA TTT GCT CTG CTG TCC TG-3′ | +60 | |||
| ex5 | 464FW | 5′-CCT CGT GAA TTA CAG CAA GC-3′ | −57 | 337 | |
| 631RV | 5′-GCA ATT CTC TTG CCT CAG TC-3′ | +112 | |||
| ex6a | 632FW | 5′-ATT CCA TAG GAC GAA GCA GC-3′ | −173 | 350 | |
| 808RV | 5′-CAT ACA TGG CGT CCA AGC CAA G-3′ | nt 808 | |||
| ex6b | 724FW | 5′-GAC TGC TCT ATT GTC TGT GGC-3′ | nt 724 | 286 | |
| 881RV | 5′-CCA TGC AAT GGT ATT CAT GGA GG-3′ | +128 | |||
| ex7 | 882FW | 5′-GAA GGC AGT AAT TAG GGA GG-3′ | −130 | 284 | |
| 955RV | 5′-TGC AAC CCA CTG AGG AAA CG-3′ | +80 | |||
| ex8a | 956FW | 5′-GTT CCG ACT TTC AGG AAC GGC-3′ | −66 | 159 | |
| 1049RV | 5′-GGG TTT CGG ACG CAG AAA GAC-3′ | nt 1049 | |||
| ex8b | 986FW | 5′-CAG CAT TTG AGG GCT GCT TGA G-3′ | nt 986 | 312 | |
| 1297RV | 5′-GTC CAA AGG GTA CAT CAG CTC C-3′ | nt 1297 | |||
| ex8c | 1235FW | 5′-CCC CTC TCA CTG ACA AAC AC-3′ | nt 1235 | 186 | |
| 1326RV | 5′-AGC CCC AAA TCC CCA TCT AGC-3′ | +94 | |||
| ex9a | 1327FW | 5′-ATT CTC TCC CTC ATC TTA GG-3′ | −137 | 309 | |
| 1390RV | 5′-CTT TCT TGT GGT CCA GCA CG-3′ | nt 1390 | |||
| ex9b | 1498FW | 5′-CTT CAA GAC ATC TGC TTG GC-3′ | nt 1498 | 294 | |
| 1553RV | 5′-GTA AAC TTC ACA GGG CAA GG-3′ | +130 | |||
| ex10 | 1554FW | 5′-AGG GCC CAT GTT GTC CTT AG-3′ | −119 | 285 | |
| 1654RV | 5′-TGA TGC TAA TGA CAA AAC CGA G-3′ | +65 | |||
| ex11 | 1655FW | 5′-GAG ATA CAG TCC ATA GCT CC-3′ | −115 | 288 | |
| 1787RV | 5′-AAG TGC TTG CCG CAA GTG TC-3′ | +70 | |||
| ex12 | 1758FW | 5′-AAG TTT CTT ACT TAG CTG TCA G-3′ | −62 | 315 | |
| 1947RV | 5′-GAC GTT ACA CTG TGC ACT GC-3′ | +63 | |||
| ex13 | 1948FW | 5′-AAG TGG GAC AGA CAA CCC TG-3′ | −56 | 303 | |
| 2130RV | 5′-GGA GCC ATT CAC AGT CCC TG-3′ | +64 | |||
| ex14 | 2131FW | 5′-CAA GGC AGC AAG AAA TGG CG-3′ | −70 | 261 | |
| 2245RV | 5′-CAT GTT CAG GTA GCC AGC TC-3′ | +76 | |||
| ex15 | 2246FW | 5′-GAA CAT AAG ACC TGC AGA GAG C-3′ | −69 | 263 | |
| 2373RV | 5′-CCG CTA GCT GCT TCC TCT AG-3′ | +66 | |||
| ex16 | 2374FW | 5′-CTA GAG GAA GCA GCT AGC GG-3′ | −51 | 279 | |
| 2514RV | 5′-TCC TTC CCA GGC TGT CTG GC-3′ | +87 | |||
| ex17 | 2515FW | 5′-TGT ACT CCC TAT TAG CCT GTC-3′ | −137 | 316 | |
| 2604RV | 5′-CTT GCT TGA AAC ACC TAC GTG C-3′ | +90 | |||
| ex18 | 2605FW | 5′-CTT ATT CTC CGT GAT CCT CGC-3′ | −80 | 355 | |
| 2795RV | 5′-CAG TGA GAC ATT TCA GGC CTG-3′ | +84 | |||
| ex19 | 2796FW | 5′-AGA CTT CCT CCC TGT GGA GC-3′ | −49 | 257 | |
| 2911RV | 5′-GGT ATA AAC TGA GGC ACG ATG C-3′ | +92 | |||
| ex20 | 2912FW | 5′-GTA ATG CCC CTC ACT GTC AG-3′ | −94 | 306 | |
| 3041RV | 5′-GTC TTA GCC CAG TCC TCT CC-3′ | +82 | |||
| ex21 | 3042FW | 5′-AAT GTA CAG CTG GGT CTG ACC-3′ | −124 | 378 | |
| 3245RV | 5′-CAG TGT AGG CCC TTT GCT GG-3′ | +50 | |||
| ex22 | 3246FW | 5′-TGT TCG GGA GTG AGA GCG AGC-3′ | −50 | 357 | |
| 3477RV | 5′-ATG GAA TCT AAG ACA GCC AAT CC-3′ | +75 | |||
| ex23 | 3478FW | 5′-AGC ACC CAT CCT CAG AAC GG-3′ | −81 | 265 | |
| 3591RV | 5′-CTC TTC AGT CAC TGA GGA GG-3′ | +69 | |||
| ex24 | 3592FW | 5′-CAA TTA CAG GTT GGT AAA AGT GG-3′ | −50 | 255 | |
| 3754RV | 5′-ATG TCC TTC CAT TGT GCC ACC-3′ | +43 | |||
| ex25 | 3755FW | 5′-TGA GCC ACT ATG CCC AGC CAA C-3′ | −81 | 188 | |
| 3870RV | 5′-GAC ACA GTT CAG TCA GGA TG-3′ | nt 3870 | |||