PCR primers to amplify exons 8 and 9 following bisulphite conversion of genomic DNA
| Primer name | Primer sequence (5′ → 3′) |
| First round degenerate primers | |
| 8-9Fa | agt ttt tgg gtt tag aat ggg g |
| 8-9Fb | agt ttt tgg gtt cag aat ggg g |
| 8-9Fc | agt ttt tgg gtc tag aat ggg g |
| 8-9Fd | agt ttt tgg gtc cag aat ggg g |
| 8-9Ra | gaa tat taa ata aac aaa cta aac acc tac |
| 8-9Rb | gaa tat taa ata aac aaa ctg aac acc tac |
| 8-9Rc | gaa tat taa ata aac aaa cta gac acc tac |
| 8-9Rd | gaa tat taa ata aac aaa ctg gac acc tac |
| 2nd round inner nested primers | |
| 8-9innerF | gcg gaa ttc ttg atg ttg att att ttt ttt ttt aat agg t |
| 8-9innerR | cgc gcg aat tcc gcg atc cta tct tta aaa aaa ac |