Primer name
|
Primer sequence (5′ → 3′)
|
First round degenerate primers
|
8-9Fa | agt ttt tgg gtt tag aat ggg g |
8-9Fb | agt ttt tgg gtt cag aat ggg g |
8-9Fc | agt ttt tgg gtc tag aat ggg g |
8-9Fd | agt ttt tgg gtc cag aat ggg g |
8-9Ra | gaa tat taa ata aac aaa cta aac acc tac |
8-9Rb | gaa tat taa ata aac aaa ctg aac acc tac |
8-9Rc | gaa tat taa ata aac aaa cta gac acc tac |
8-9Rd | gaa tat taa ata aac aaa ctg gac acc tac |
2nd round inner nested primers |
8-9innerF | gcg gaa ttc ttg atg ttg att att ttt ttt ttt aat agg t |
8-9innerR | cgc gcg aat tcc gcg atc cta tct tta aaa aaa ac |