Table 1

PCR primers to amplify exons 8 and 9 following bisulphite conversion of genomic DNA

Primer name Primer sequence (5′ → 3′)
First round degenerate primers
8-9Faagt ttt tgg gtt tag aat ggg g
8-9Fbagt ttt tgg gtt cag aat ggg g
8-9Fcagt ttt tgg gtc tag aat ggg g
8-9Fdagt ttt tgg gtc cag aat ggg g
8-9Ragaa tat taa ata aac aaa cta aac acc tac
8-9Rbgaa tat taa ata aac aaa ctg aac acc tac
8-9Rcgaa tat taa ata aac aaa cta gac acc tac
8-9Rdgaa tat taa ata aac aaa ctg gac acc tac
2nd round inner nested primers
8-9innerFgcg gaa ttc ttg atg ttg att att ttt ttt ttt aat agg t
8-9innerRcgc gcg aat tcc gcg atc cta tct tta aaa aaa ac