Table 2

Single nucleotide polymorphic markers used

SNP Primers
+9 of 3′ end of exon 1Fw GACCGGGGCGACTTCTATAC
c→g at 211+9Rev CACTCCGTGATCACAAGTTCAG
−9 of 5′ end of exon 10Fw AAACTAACATTCATAAGGGAGTTAAGG
t→a at 1511-9Rev CCAAACTGTGCACTGGAATC
+12 of 3′ end of exon 10Fw TGTAAGGAAGAAAAAGTCCTTCG
g→a at 1661+12Rev GAAAGCTTGACTCTTACCTGATGAC
+3 of 3′ end of exon 5Fw TGTAAAACGACGGCCAGTGTGGTATAGAAATCTTCGATTTTT
A→T at nt942+3Rev CAGGAAACAGCTATGACCAATCAACATTTTTAACCCTTTT
Exon 2 codon 113Fw TGTAAAACGACGGCCAGTGTCCAGCTAATACAGTGCTTG
AAG→AAARev CAGGAAACAGCTATGACCCACATTTTTATTTTTCTACTCTTAA
Exon 2 codon 110Fw TGTAAAACGACGGCCAGTGTCCAGCTAATACAGTGCTTG
A→G at 329Rev CAGGAAACAGCTATGACCCACATTTTTATTTTTCTACTCTTAA
Exon 2 codon 96Fw TGTAAAACGACGGCCAGTGTCCAGCTAATACAGTGCTTG
G→A at 287Rev CAGGAAACAGCTATGACCCACATTTTTATTTTTCTACTCTTAA
Exon 3 codon 133FwTGTAAAACGACGGCCAGTAAAGTATGTTCAAGAGTTTGTTAAATTTTT
C→T at 399Rev CAGGAAACAGCTATGACCCTTTCCTAGGCCTGGAATCTC
Exon 3 codon 153FwTGTAAAACGACGGCCAGTAAAGTATGTTCAAGAGTTTGTTAAATTTTT
C→T at 459Rev CAGGAAACAGCTATGACCCTTTCCTAGGCCTGGAATCTC
Exon 6 codon 322Fw TGTAAAACGACGGCCAGTTTTTCACTAATGAGCTTGCCATTCTT
G→A at 965Rev CAGGAAACAGCTATGACCGGTAACTGCAGGTTACATAAAACTAACGA
Exon 6 codon 328Fw TGTAAAACGACGGCCAGTTTTTCACTAATGAGCTTGCCATTCTT
GCC→GCTRev CAGGAAACAGCTATGACCGGTAACTGCAGGTTACATAAAACTAACGA
−10 of 5′ end of exon 7Fw TGTAAAACGACGGCCAGTCGACTTAGTTGAGACTTACGTGC
t→c at 1077-10Rev CAGGAAACAGCTATGACCTTTATGAGGACAGCACATTGC
+49 of 3′ end of exon 7Fw TGTAAAACGACGGCCAGTCGACTTAGTTGAGACTTACGTGC
c→a at 1276+49Rev CAGGAAACAGCTATGACCTTTATGAGGACAGCACATTGC
Exon 11 codon 556Fw TGTAAAACGACGGCCAGTCATTGCTTCTAGTACACATTT
T→C at 1666Rev CAGGAAACAGCTATGACCCAGGTGACATTCAGAACATTA
Exon 11 codon 579Fw TGTAAAACGACGGCCAGTCATTGCTTCTAGTACACATTT
A→G at 1737Rev CAGGAAACAGCTATGACCCAGGTGACATTCAGAACATTA
Exon 11 codon 585Fw TGTAAAACGACGGCCAGTCATTGCTTCTAGTACACATTT
TCT→TCCRev CAGGAAACAGCTATGACCCAGGTGACATTCAGAACATTA
Exon 12 codon 596Fw TGTAAAACGACGGCCAGTTCAGTATTCCTGTGTACATTT
A→G at 1787Rev CAGGAAACAGCTATGACCTTACCCCCACAAAGCCCAA
−6 of 5′ end of exon 13Fw TGTAAAACGACGGCCAGTGTAGCAGAAAGAAGTTTAAAACTTGC
t→c at 2006-6Rev CAGGAAACAGCTATGACCGGACAGAGACATACATTTCTATCTTC
Exon 13 codon 713Fw TGTAAAACGACGGCCAGTGTAGCAGAAAGAAGTTTAAAACTTGC
G→C at 2139Rev CAGGAAACAGCTATGACCGGACAGAGACATACATTTCTATCTTC
Exon 13 codon 718Fw TGTAAAACGACGGCCAGTGTAGCAGAAAGAAGTTTAAAACTTGC
A→G at 2154Rev CAGGAAACAGCTATGACCGGACAGAGACATACATTTCTATCTTC
MicrosatellitesPrimers
TTTA repeatFw FAM-GAGGATGGCCACAAATTAGC2-150
Rev ACCAGGGAGTCAGAGTTTGC
TTTG repeatFw NED-TGGTGTAGGCAGCCATGTATC2-150
Rev CCTCTGCCTAGGAAAACCAG
TAAA repeatFw HEX-TGAGTATTGCTCTCTTGCTATCTTG2-150
Rev AGAGCCGTAATCACTCAATGTG
CA repeat 1Fw FAM-TCACCCCAGCCAGACTCTAAG2-150
Rev GTTTCTTCAGATTTTTATTGAGAACCTACCAGG
CA repeat 2Fw FAM-TGTTGGACTCCGCAGGATTG2-150
Rev GTTTCTTTAGGTGTATGTAGTAGAGGGCAAGC
CA repeat 3Fw HEX-GTCTCTCTCTGTGTGTCTTTCTGCC2-150
Rev GTTTCTTGCCAACTGGTTCCATTTGACC
  • 2-150 FAM, NED, and HEX are fluorescent labels tagged onto the 5′ end of the custom primers.