Excel macro sheet of UPQFM-PCR of exons 3, 5, 8, 14, and 17 of LDLR
| Normals | Peak areas of controls | |||||
|---|---|---|---|---|---|---|
| Exons | C1 | C2 | C3 | C4 | ||
| 3 | ||||||
| 5 | ||||||
| 8 | ||||||
| 14 | ||||||
| 17 | ||||||
| Normals | Mean 2 SD of exon peak area ratios | |||||
| Exons | 3 | 5 | 8 | 14 | 17 | |
| 3 | (ex3/3) | (ex3/5) | (ex3/8) | (ex3/14) | (ex3/17) | |
| 5 | (ex5/5) | (ex5/8) | (ex5/14) | (ex5/17) | ||
| 8 | (ex8/8) | (ex8/14) | (ex8/17) | |||
| 14 | (ex14/14) | (ex14/17) | ||||
| 17 | (ex17/17) | |||||
| Areas | Ratio of peak areas | |||||
| Exons | 3 | 5 | 8 | 14 | 17 | |
| 3 | ex3/3 | ex3/5 | ex3/8 | ex3/14 | ex3/17 | |
| 5 | ex5/5 | ex5/8 | ex5/14 | ex5/17 | ||
| 8 | ex8/8 | ex8/14 | ex8/17 | |||
| 14 | ex14/14 | ex14/17 | ||||
| 17 | ex17/17 | |||||
| Results | ||||||
Ratios are determined from the peak area under each exon against the other exons in the multiplex assay. The mean (1 and 2 SD) of the ratios of four normal controls are calculated. Exon peak area ratios of the patients are then compared with these confidence limits.