Oligonucleotides for the UPQFM-PCR
| Exon | Oligonucleotide sequence (sense & antisense 5′-3′) | PCR size (bp) | ||
| P1 Univ | F: TCC GTC TTA GCT GAG TGG CGT A TC CCC G | — | ||
| R: AGG CAG AAT CGA CTC ACC GCT A TC CAC G | ||||
| 3 | F: P1UnivF-CC TTT GAG TGA CAG TTC AAT CCT G | 261 | ||
| R: P1UnivR-CC CAG GAC TCA GAT AGG CTC AAT A | ||||
| 5 | F: P1UnivF-GA GAA AAT CAA CAC ACT CTG TCC TG | 239 | ||
| R: P1UnivR-GA AAA CCA GAT GGC CAG CG | ||||
| 8 | F: P1UnivF-GT CTC TAG GCC ATT GGG GAA | 285 | ||
| R: P1UnivR-AC TGC TGC CTG CAA GGG GT | ||||
| 14 | F: P1UnivF-GG AAT GTT CTG GAA ATT TCT GGA AT | 322 | ||
| R: P1UnivR-AC ACG GGA CGC AGA AAC AAG GC | ||||
| 17 | F: P1UnivF-GT GCC TCT CCC TAC AGT GCT CCT | 223 | ||
| R: P1UnivR-TC CAC GGT AGC TGT AGC CGT CCT GGT TGT | ||||
| P2 Univ | F: TCC GTC TTA GCT GAG TGG CGT A | — | ||
| R: 6-Fam-AGG CAG AAT CGA CTC ACC GCT A | ||||
The P1 universal primer sequence (underlined) is attached to the 5′ end of a six base tag (bold) (described in Materials and methods). The fragment sizes include the universal-unique primer sequences. The universal primer sequence common between the P1 and P2 primers is underlined. A fluorescent label (6-Fam) is attached to the 5′ end of the antisense P2 universal primer.