Table 1

ESTs tested in this study and results of the PCR screening

EST		Name		Map position		Forward primer		Reverse primer		GM09189
EST		Name		Map position		Forward primer		Reverse primer		11	17	18	C	(PWS)	A	AM
WI-9340	ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, isoform1	98-102 cM	TGAGAGAACGAAATCTCTATCGG	AGGCAGCAAGTTTTTATAAAGGC	+	+	+	+	NT	+	+
stSG4756	Highly similar to cerebellin precusor	102-109 cM	TCCTTTTTGATTGCTTTGGG	AGAAGCCCACCTGAAAGTCA	+	+	+	+	NT	+	+
stSG1384	Hypothetical 52.9 kDa protein in CDC26-YMR31 intergenic region	109-118 cM	CCAGTTAGTGAGGTTTTGAGGG	GGCCTCCTCTGATCTCTCG	+	+	+	+	NT	+	+
A004F30	Similar to probable E1-E2 ATPase	109-118 cM	CTTGACTGTTTTTACTGAAAAG	CTGAGAATTAGACTGAAAGTTTA	−	−	−	−	NT	+	+
SHGC-11871	Nuclear factor of activated T cells (NF-ATc)	117-123 cM	ATGTCCCGTTAGTGAGACCG	AAGCAGCTTTAGGGTGCAAA	+	+	+	+	NT	+	+
hGR1/2	Galanin receptor 1	>123 cM	CTGGCCTACCTGCTCTTCTG	GAAGAGGCCCTGGTGGTAG	−	−	−	−	NT	+	+
WI-9286	Golli/myelin basic protein	>123 cM	AGGTCTCGTTCCGTGCTG	GCCACCATCCCTTGTGAG	+	+	+	+	NT	+	+
CYT5B.A/B	Cytochrome b-5	18q23	TCCATCCAGATGACAGACCA	CTGCCCATGTTATAGGCGAT	+	+	+	+	NT	+	+
SGC32075	Unidentified transcript	109 cM	TATTTATTTACCACGCCTGTTTC	CCTGTGTCAGAAGAGCACAA	+	+	+	+	NT	+	+
WI-6843	Unidentified transcript	109 cM	ACATGGTGTAACATTTCCAATAACA	TGGACTCGCTACTCCGTTCT	+	+	+	+	+	+	+
IB2403	Unidentified transcript	102-109 cM	TGGCATAGAAAAGGGTAAAA	GGCAGCTCTCCAGAAGTAAT	+	+	+	+	NT	+	+
WI-15273	Unidentified transcript	109-117 cM	TCCTGTTGCTCCCAGTCC	TGGGAGATCAAGAGTCCTCG	−	−	−	−	NT	+	+
H81050	Unidentified transcript	109-118 cM	TTTGACACTACCAGGTTCTATGAC	GGGCACACTACTCAGAGACATT	+	+	+	+	+	+	+
stSG3734	Unidentified transcript	109-118 cM	TCAACTTATGTGTGCTGCTGG	TACCCAAGAACGTGTGGTGA	−	−	−	−	NT	+	+
stSG2738	Unidentified transcript	109-118 cM	CGTCGACCCCAGTTTTTG	AGTGCGTGCTTTGTCCATC	−	−	−	−	NT	+	+
SGC32005	Unidentified transcript	118-123 cM	GCAATCGGCGACAGTTTAAT	TGAATGTCTGAGTGAGGGTCC	+	+	+	+	NT	+	+
Cda0ea07	Unidentified transcript	118-123 cM	CTGTCCTTGGTCTGTTAAACTG	TGATGGTAATTGCCTGTGGTG	+	+	+	+	NT	+	+
A004B28	Unidentified transcript	123-124 cM	CCTAAAATTATGAATAGCACAC	GAAGGAAGGTTATATTTCTTATC	−	−	−	−	NT	+	+
A007H41	Unidentified transcript	123-124 cM	CTCTCTGAGCTTCAGTTTC	TCTTCCCATAAATGACAG	−	−	−	−	NT	+	+
SGC31888	Unidentified transcript	123-124 cM	GAGTTTAATTGAGCAAAGAATGATT	GCTGTTTGCAGGCCTCAC	+	+	+	+	NT	+	+
SGC30781	Unidentified transcript	123-124 cM	AAAGTTGTCGCACTGAATAAGAG	GACAAGTGAACAAGATCAATTTTTT	−	−	−	−	NT	+	+
WI-15059	Unidentified transcript	123-124 cM	CTCTCCTAGGGGGTGATGC	GCGAGCCCGTTAGTGATG	−	−	−	−	NT	+	+

11 = cDNA derived from a lymphoblastoid cell line of patient 11 with a paternal deletion. 17 and 18 = cDNAs derived from lymphoblastoid cell lines of patients 17 and 18 with a maternal and a paternal deletion, respectively.1 C = control cDNA derived from a lymphoblastoid cell line of a person with a normal chromosome 18. A and AM = genomic DNA of normal subjects. NT = not tested.