Table 1

Primer pairs used in PCR amplification of the seven candidate gene coding regions

Primer pairs	Forward primer (5′-3′)	Reverse primer (5′-3′)	Annealing temp (°C)
UBE1-1	CAGTCCGTGTTGTCCGA	GCCCGGTTTTTACCGATG	55
UBE1-2	AGGAGGACATCGGTAAAAC	ACTTGGCGAAGTCCGTCA	55
UBE1-3	GACTTTGTGGTGACGGAC	GTGTCCATGTCTGTAACGA	55
UBE1-4	AGGGTGGAGAAATCATCG	CTGCCGGATGTTGTTCGA	55
UBE1-5	CACCCAGTACTCGAACAA	GCTCTGTCTTAAAATAGTCG	55
UBE1-6	TCAAACAGTTCCTCGACTATT	GCGGATGGTGTATCGGAC	55
UBE2I	TCCACCTGTCCGCTACGCT	CAATCCCTTCCTTCTGACGAT	59
GdX1-1	ACTTGGCCCGACTGGTTCCT	CCGCACCCCGACCCGACACA	62
GdX1-2	AGTGGACGCCGTCGCGAC	ACACACGGCTGCTGGCCTC	60
GdX2	AGGAGGAACCCATCGATCT	AACACACTTAGTGCGACATGC	57
SOX3-1	AGCTAGAGCCCAGGCAGACT	CTTTCTTGAGCAGCGTCTTG	59
SOX3-2	CCGGACTACAAGTACCGACC	GCTGAGAGTGCGATGCGATG	59
SOX3-3	CCATGGGCTCGGTAGTGAAGT	GTGGGGAACAAGGGTGGAC	59
GABRA3-1-1	CATCCTTCTCTCCTTTCCGCT	GTGTCCGGTGTCCAGATCTT	55
GABRA3-1-2	CTCAGACTGTAGAGCTCGGT	GATTGTTCAGTGGAAGGATC	58
GABRA3-2-1	AGTGAAGACTGACATCTACGTG	GGCACTGATACTCAAGGTG	55
GABRA3-2-2	CAGACATGGCATGATGAAAGA	TGTACGGGCAGGAACAGAC	57
GABRA3-3	TGGCTACTTTGTGATCCAGA	GATGCTTCACGGGGTATAC	57
CDR2-1	GCCGAGATGCTGGCGGAAA	GATGACTTCAGCTCCTCCACT	62
CDR2-2	AGATTCTGAGCCTGACTGAAA	AACATACAGAGAGTCTGGCAC	59
CDR2-3	AGGCCGAGGTCGCAGAGATG	AGAGAGGCGATAGGCAATAGG	62
MECP2-1	ACACACACTGACCTTTCAGG	AATGTCCCAAATAGCCCTGG	60
MECP2-2	CCTGGGCACATACATTTTCCT	TGGCATGTTCTCTGTGATACTT	60
MECP2-3-1	CCCACCCTGAAGCCACGAAACT	CCTGCCCCCACCTCCACCTG	67
MECP2-3-2	CTGCTGCTCAAGTCCTGGGGCT	CGACCCTCAGGCCATTCCCAAGA	68
MECP2-3-3	TCCTTCACGGCTTTCTTTTTGG	TCCTTGTGTCTTTCTGTTTGTC	60