Table 1

Sequences of the primers

Exon	Forward primer	Reverse primer	Product size (bp)	Annealing temp (°C)
1a	GCCCCCGCCGTAGCAGT	ACGAAGAGGAGCCCCAGGTG	318	62^1-150
1b	TACTATTTCTCCTACCCGCTGTTT	CGACCCCACCGCCTTCT	321	57^1-150
1c	CGGCGGCGGCAGTGAGAG	ATGAGGGCGCGGGAGAAG	284	62^1-150
2	TTTTTATGTATTACCTCTCAAC	TGGGATGGCTATAAACAAAT	204	55
3	TTAGTTGGGAAATGTAGAT	TATGTTAAAAAGCCTGGAC	246	55
4	TTTTTACCTTCTCTGTTG	AAGCTTTATTATTTTATGTTAGTT	156	55
5	GTTCAGCTACAATTTTCTAATCAC	TATGATCAACTTAAGCAGGAAT	321	55
6	ATGTTAGGTTGTATTTTCA	CAAGGTATTTATTATCTATTTCAC	273	55
7	TCATAGGGCTTAGGCTTCA	ATGGATTCAGTAACAGATGGTATT	224	55
8	CTGTTTGGGAAGATGCT	CTCAAGGACAAGATAAAGTT	261	60
9	TGGCCTCATAGCTTACATTTTTAG	ATACGACAATATTGGAAACAGAG	220	55
10	GTGCTAGATTTTCAACATA	GCCCTTCTTTAAAACTTCTTCC	270	55
11	GAATTTAGTAGGACCCACT	GCCACATTAAAAATATCATA	230	55
12	ATGGCCAAGGTTAAAAATACAA	CTGGAAGAAAATAGTGAAT	281	55
13	CTTTTCCTGTCATTTGCTGTTT	GATGGTAGTTCTTGTTTCTGCTCT	173	55
14	ATCATTAATTCTGAAATTAG	ATAAACCAAATCCAAA	154	48
15	AAAAAGCGGGAGGGGAAATA	TGGGCAACAGAGTGAGACC	248	56^1-150
16	TGTATGTATTTTTAAGTGCCTGAC	TTACAATATAGAAGACAAAGAAA	125	55
17	AACAGCAGCATCATTACTTT	GTTCTGCAGGTTTACAA	241	55

↵1-150 Requires the addition of 2 mol/l Betaine (Sigma) to the PCR reaction.