PT - JOURNAL ARTICLE AU - Moreno-Cabrera, José Marcos AU - del Valle, Jesús AU - Feliubadaló, Lidia AU - Pineda, Marta AU - González, Sara AU - Campos, Olga AU - Cuesta, Raquel AU - Brunet, Joan AU - Serra, Eduard AU - Capellà, Gabriel AU - Gel, Bernat AU - Lázaro, Conxi TI - Screening of CNVs using NGS data improves mutation detection yield and decreases costs in genetic testing for hereditary cancer AID - 10.1136/jmedgenet-2020-107366 DP - 2022 Jan 01 TA - Journal of Medical Genetics PG - 75--78 VI - 59 IP - 1 4099 - http://jmg.bmj.com/content/59/1/75.short 4100 - http://jmg.bmj.com/content/59/1/75.full SO - J Med Genet2022 Jan 01; 59 AB - Introduction Germline CNVs are important contributors to hereditary cancer. In genetic diagnostics, multiplex ligation-dependent probe amplification (MLPA) is commonly used to identify them. However, MLPA is time-consuming and expensive if applied to many genes, hence many routine laboratories test only a subset of genes of interest.Methods and results We evaluated a next-generation sequencing (NGS)-based CNV detection tool (DECoN) as first-tier screening to decrease costs and turnaround time and expand CNV analysis to all genes of clinical interest in our diagnostics routine. We used DECoN in a retrospective cohort of 1860 patients where a limited number of genes were previously analysed by MLPA, and in a prospective cohort of 2041 patients, without MLPA analysis. In the retrospective cohort, 6 new CNVs were identified and confirmed by MLPA. In the prospective cohort, 19 CNVs were identified and confirmed by MLPA, 8 of these would have been lost in our previous MLPA-restricted detection strategy. Also, the number of genes tested by MLPA across all samples decreased by 93.0% in the prospective cohort.Conclusion Including an in silico germline NGS CNV detection tool improved our genetic diagnostics strategy in hereditary cancer, both increasing the number of CNVs detected and reducing turnaround time and costs.