RT Journal Article SR Electronic T1 Exosomes derived from exhausted CD8+ T cells impaired the anticancer function of normal CD8+ T cells JF Journal of Medical Genetics JO J Med Genet FD BMJ Publishing Group Ltd SP 29 OP 31 DO 10.1136/jmedgenet-2018-105439 VO 56 IS 1 A1 Wang, Xiaochen A1 Shen, Haiyuan A1 He, Qifeng A1 Tian, Wenfang A1 Xia, Anliang A1 Lu, Xiao-Jie YR 2019 UL http://jmg.bmj.com/content/56/1/29.abstract AB Background Previous studies suggested that diverse cells in cancer microenvironment can interact with CD8+ T cells via exosomes. We designed this study to explore the potential interaction between exhausted CD8+ T cells and normal CD8+ T cells via exosome.Methods Fluorescence activated cell sorting was used to get PD1+TIM3+/PD1−TIM3−CD8+ T cells. Exosomes from the cell culture medium were collected by ultracentrifugation. Microarrays were performed to analyse the lncRNA expression profile in exosomes.Results Functional exhausted CD8+ T cells could secrete vast exosomes, which can be uptake by normal CD8+ T cells, and impaired their proliferation (Ki67), cell activity (CD69) and the production of cytokines such as interferon-γ and interleukin-2. Microarray detection identified 257 candidate lncRNAs differently expressed in exosomes derived from exhausted CD8+ T cells and non-exhausted CD8+ T cells. Functional enrichment analysis indicated that these lncRNAs actively participated in the regulation of diverse process of CD8+ T cell activity, like metabolism, gene expression, biosynthetic process and so forth.Conclusions The exosomes derived from exhausted CD8+ T cells could be uptake by non-exhausted CD8+ T cells and subsequently impaired the function of receipt cells. Exosomes secreted from exhausted CD8+ T cells have distinct lncRNA expression profiles which are significantly different from those in exosomes secreted by non-exhausted CD8+ T cells.