RT Journal Article
SR Electronic
T1 Exosomes derived from exhausted CD8+ T cells impaired the anticancer function of normal CD8+ T cells
JF Journal of Medical Genetics
JO J Med Genet
FD BMJ Publishing Group Ltd
SP 29
OP 31
DO 10.1136/jmedgenet-2018-105439
VO 56
IS 1
A1 Xiaochen Wang
A1 Haiyuan Shen
A1 Qifeng He
A1 Wenfang Tian
A1 Anliang Xia
A1 Xiao-Jie Lu
YR 2019
UL http://jmg.bmj.com/content/56/1/29.abstract
AB Background Previous studies suggested that diverse cells in cancer microenvironment can interact with CD8+ T cells via exosomes. We designed this study to explore the potential interaction between exhausted CD8+ T cells and normal CD8+ T cells via exosome.Methods Fluorescence activated cell sorting was used to get PD1+TIM3+/PD1−TIM3−CD8+ T cells. Exosomes from the cell culture medium were collected by ultracentrifugation. Microarrays were performed to analyse the lncRNA expression profile in exosomes.Results Functional exhausted CD8+ T cells could secrete vast exosomes, which can be uptake by normal CD8+ T cells, and impaired their proliferation (Ki67), cell activity (CD69) and the production of cytokines such as interferon-γ and interleukin-2. Microarray detection identified 257 candidate lncRNAs differently expressed in exosomes derived from exhausted CD8+ T cells and non-exhausted CD8+ T cells. Functional enrichment analysis indicated that these lncRNAs actively participated in the regulation of diverse process of CD8+ T cell activity, like metabolism, gene expression, biosynthetic process and so forth.Conclusions The exosomes derived from exhausted CD8+ T cells could be uptake by non-exhausted CD8+ T cells and subsequently impaired the function of receipt cells. Exosomes secreted from exhausted CD8+ T cells have distinct lncRNA expression profiles which are significantly different from those in exosomes secreted by non-exhausted CD8+ T cells.