RT Journal Article
SR Electronic
T1 MEIS1 and BTBD9: genetic association with restless leg syndrome in end stage renal disease
JF Journal of Medical Genetics
JO J Med Genet
FD BMJ Publishing Group Ltd
SP 462
OP 466
DO 10.1136/jmg.2010.087858
VO 48
IS 7
A1 Schormair, Barbara
A1 Plag, Jens
A1 Kaffe, Maria
A1 Groß, Nadine
A1 Czamara, Darina
A1 Samtleben, Walter
A1 Lichtner, Peter
A1 Ströhle, Andreas
A1 Stefanidis, Ioannis
A1 Vainas, Andreas
A1 Dardiotis, Efthimios
A1 Sakkas, George K
A1 Gieger, Christian
A1 Müller-Myhsok, Bertram
A1 Meitinger, Thomas
A1 Heemann, Uwe
A1 Hadjigeorgiou, Georgios M
A1 Oexle, Konrad
A1 Winkelmann, Juliane
YR 2011
UL http://jmg.bmj.com/content/48/7/462.abstract
AB Background Restless legs syndrome (RLS) is a sleep related movement disorder that occurs both in an idiopathic form and in symptomatic varieties. RLS is a frequent and distressing comorbidity in end stage renal disease (ESRD). For idiopathic RLS (iRLS), genetic risk factors have been identified, but their role in RLS in ESRD has not been investigated yet. Therefore, a case–control association study of these variants in ESRD patients was performed.Methods The study genotyped 10 iRLS associated variants at four loci encompassing the genes MEIS1, BTBD9, MAP2K5/SKOR1, and PTPRD, in two independent case–control samples from Germany and Greece using multiplex PCR and MALDI-TOF (matrix assisted laser desorption/ionisation time-of-flight) mass spectrometry. Statistical analysis was performed as logistic regression with age and gender as covariates. For the combined analysis a Cochran–Mantel–Haenszel test was applied.Results The study included 200 RLS-positive and 443 RLS-negative ESRD patients in the German sample, and 141 and 393 patients, respectively, in the Greek sample. In the German sample, variants in MEIS1 and BTBD9 were associated with RLS in ESRD (Pnom≤0.004, ORs 1.52 and 1.55), whereas, in the Greek sample, there was a trend for association to MAP2K5/SKOR1 and BTBD9 (Pnom≤0.08, ORs 1.41 and 1.33). In the combined analysis including all samples, BTBD9 was associated after correction for multiple testing (Pcorrected=0.0013, OR 1.47).Conclusions This is the first demonstration of a genetic influence on RLS in ESRD patients with BTBD9 being significantly associated. The extent of the genetic predisposition could vary between different subgroups of RLS in ESRD.