RT Journal Article
SR Electronic
T1 Altered CD45 expression in C77G carriers influences immune function and outcome of hepatitis C infection
JF Journal of Medical Genetics
JO J Med Genet
FD BMJ Publishing Group Ltd
SP 678
OP 684
DO 10.1136/jmg.2005.040485
VO 43
IS 8
A1 R Dawes
A1 B Hennig
A1 W Irving
A1 S Petrova
A1 S Boxall
A1 V Ward
A1 D Wallace
A1 D C Macallan
A1 M Thursz
A1 A Hill
A1 W Bodmer
A1 P C L Beverley
A1 E Z Tchilian
YR 2006
UL http://jmg.bmj.com/content/43/8/678.abstract
AB Background: A polymorphism in exon 4 (C77G) of CD45 that alters CD45 splicing has been associated with autoimmune and infectious diseases in humans. Objective: To investigate the effect of C77G in hepatitis C virus (HCV) infected individuals and study the phenotype and function of peripheral blood mononuclear cells (PBMC) from healthy and hepatitis C infected C77G carriers. Results: C77G individuals showed an increased proportion of primed CD45RA and effector memory CD8 T cells and more rapid activation of the lymphocyte specific protein tyrosine kinase (Lck) following CD3 stimulation. Transgenic mice with CD45 expression mimicking that in human C77G variants had more activated/memory T cells, more rapid proliferative responses, and activation of Lck. Conclusions: Changes in CD45 isoform expression can alter immune function in human C77G variants and CD45 transgenic mice. The C77G allele may influence the outcome of HCV infection.