PT  - JOURNAL ARTICLE
AU  - Engelen, J J
AU  - Albrechts, J C
AU  - Hamers, G J
AU  - Geraedts, J P
TI  - A simple and efficient method for microdissection and microFISH.
AID  - 10.1136/jmg.35.4.265
DP  - 1998 Apr 01
TA  - Journal of Medical Genetics
PG  - 265--268
VI  - 35
IP  - 4
4099  - http://jmg.bmj.com/content/35/4/265.short
4100  - http://jmg.bmj.com/content/35/4/265.full
SO  - J Med Genet1998 Apr 01; 35
AB  - A simple and efficient method for the dissection of (marker) chromosomes, (micro)nuclei, and chromosome regions is presented. Before microdissection, metaphases are overlaid with milli-Q water to rehydrate the chromosomes, which makes them soft and sticky. The dissected chromosome fragments are dissolved without proteinase-K or topoisomerase treatment and directly amplified using a degenerate oligonucleotide primed polymerase chain reaction (DOP-PCR). The advantages of this microFISH method over previously reported methods are: (1) microdissection in this way is very fast; (2) a chromosome, marker, (micro)nucleus, or chromosome region is collected as a whole using only one microneedle; (3) the dissected material sticks tightly to the needle without the risk of getting lost; (4) no Sequenase is used in the DOP-PCR reaction which reduces the risk of contamination.