PT - JOURNAL ARTICLE AU - Dianzani, I AU - Camaschella, C AU - Saglio, G AU - Ferrero, G B AU - Ramus, S AU - Ponzone, A AU - Cotton, R G TI - Molecular analysis of contiguous exons of the phenylalanine hydroxylase gene: identification of a new PKU mutation. AID - 10.1136/jmg.30.3.228 DP - 1993 Mar 01 TA - Journal of Medical Genetics PG - 228--231 VI - 30 IP - 3 4099 - http://jmg.bmj.com/content/30/3/228.short 4100 - http://jmg.bmj.com/content/30/3/228.full SO - J Med Genet1993 Mar 01; 30 AB - A modified application of the chemical cleavage of mismatch (CCM) method has been used to screen three contiguous exons (exons 9, 10, and 11) of the phenylalanine hydroxylase gene in 17 Italian PKU patients. A new nonsense heterozygous C-->G transversion within exon 11 (S359X) was identified in a single patient. Only one of the four mutations previously reported in this DNA region in Caucasians was found. This lesion, IVS X-546, was detected in five of the 34 PKU alleles examined. Our results underline the versatility of the CCM method for scanning a gene for multiple mutations.