RT Journal Article
SR Electronic
T1 Globin chain synthesis in sickle beta-thalassaemic bone marrow and reticulocytes.
JF Journal of Medical Genetics
JO J Med Genet
FD BMJ Publishing Group Ltd
SP 296
OP 301
DO 10.1136/jmg.16.4.296
VO 16
IS 4
A1 Ladas, S
A1 Chalevelakis, G
A1 Lyberatos, C
A1 Vaidakis, E
A1 Arapakis, G
YR 1979
UL http://jmg.bmj.com/content/16/4/296.abstract
AB Globin chain synthesis was studied in reticulocytes and bone marrow erythroid precursors in four sickle beta-thalassaemic Greek patients. Significant globin chain imbalance was found in reticulocytes (alpha/gamma + beta A + beta S = 2.20 +/- SD 0.16) and bone marrow (alpha/gamma + beta A + beta S = 1.58 +/- SD 0.11) after two hours' incubation. There was evidence of contamination of the gamma, beta A, and, to a lesser extent, of the beta S chain by non-haem proteins. The contamination was more obvious in chromatograms obtained from whole cell bone marrow samples and could partially explain the lower alpha/non-alpha ratio found in bone marrow.