PT  - JOURNAL ARTICLE
AU  - Ladas, S
AU  - Chalevelakis, G
AU  - Lyberatos, C
AU  - Vaidakis, E
AU  - Arapakis, G
TI  - Globin chain synthesis in sickle beta-thalassaemic bone marrow and reticulocytes.
AID  - 10.1136/jmg.16.4.296
DP  - 1979 Aug 01
TA  - Journal of Medical Genetics
PG  - 296--301
VI  - 16
IP  - 4
4099  - http://jmg.bmj.com/content/16/4/296.short
4100  - http://jmg.bmj.com/content/16/4/296.full
SO  - J Med Genet1979 Aug 01; 16
AB  - Globin chain synthesis was studied in reticulocytes and bone marrow erythroid precursors in four sickle beta-thalassaemic Greek patients. Significant globin chain imbalance was found in reticulocytes (alpha/gamma + beta A + beta S = 2.20 +/- SD 0.16) and bone marrow (alpha/gamma + beta A + beta S = 1.58 +/- SD 0.11) after two hours' incubation. There was evidence of contamination of the gamma, beta A, and, to a lesser extent, of the beta S chain by non-haem proteins. The contamination was more obvious in chromatograms obtained from whole cell bone marrow samples and could partially explain the lower alpha/non-alpha ratio found in bone marrow.