PT  - JOURNAL ARTICLE
AU  - R E Priest
AU  - J H Priest
AU  - J F Moinuddin
AU  - A J Keyser
TI  - Differentiation in human amniotic fluid cell cultures: I: Collagen production.
AID  - 10.1136/jmg.14.3.157
DP  - 1977 Jun 01
TA  - Journal of Medical Genetics
PG  - 157--162
VI  - 14
IP  - 3
4099  - http://jmg.bmj.com/content/14/3/157.short
4100  - http://jmg.bmj.com/content/14/3/157.full
SO  - J Med Genet1977 Jun 01; 14
AB  - The collagen produced by differentiated cells cultured from human amniotic fluid was characterized in two ways. By chain composition and by 4-hydroxyproline:3-hydroxyproline isomer ratio, the collagen synthesized by F-type (fibroblast) cells was indistinguishable from that made by cultured fetal dermal fibroblasts. The predominant cells in young amniotic fluid cultures, termed AF-type, produced collagen with a lower isomer ratio, resembling that of basement membrane collage. The chain composition, as determined by chromatography on carboxymethyl cellulose, varied for different cultures of the AF-type, but the major pattern was consistent with that of basement membrane collagen. On the basis of these characteristics, F cells are of fibroblast origin, whereas most AF cells are of a different origin either endothelial or epithelial. Other evidence (Megaw et al., 1977) suggests an epithelial origin for AF cells.