RT Journal Article
SR Electronic
T1 Glucose-6-phosphate dehydrogenase (G6PD) activity of human sperm.
JF Journal of Medical Genetics
JO J Med Genet
FD BMJ Publishing Group Ltd
SP 250
OP 255
DO 10.1136/jmg.14.4.250
VO 14
IS 4
A1 Sarkar, S
A1 Nelson, A J
A1 Jones, O W
YR 1977
UL http://jmg.bmj.com/content/14/4/250.abstract
AB Sperm from normal human donors and a G6PD-A- individual were examined for x-linked glucose-6-phosphate dehydrogenase activity and autosomally linked 6-phosphogluconate dehydrogenase activity. With the use of fluorescence microscopy, we divised a procedure to visualise in individual sperm cells the fluorescence of reduced coenzyme NADPH formed by each of the two enzymes in the presence of appropriate substrates. We found significant differences in the population distribution of sperm expressing each of the two activities, and the ratio of the two activities in sperm homogenate is very different from the one found in erythrocyte lysates. The possibility of haploid gene expression has been considered in interpreting these results.