RNA-guided engineered nucleases (RGENs) derived from the prokaryotic adaptive immune
system known as CRISPR (clustered, regularly interspaced, short palindromic repeat)/Cas …

RNA-guided endonucleases (RGENs), derived from the prokaryotic adaptive immune
system known as CRISPR/Cas, enable targeted genome engineering in cells and …

Bacteria and archaea have evolved adaptive immune defenses, termed clustered regularly
interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems, that …

Human genome engineering has been transformed by the introduction of the CRISPR
(clustered regularly interspaced short palindromic repeats)/Cas (CRISPR‐associated) …

Genome editing using the CRISPR/Cas9 system requires the presence of guide RNAs
bound to the Cas9 endonuclease as a ribonucleoprotein (RNP) complex in cells, which …

Targeted nucleases are powerful tools for mediating genome alteration with high precision.
The RNA-guided Cas9 nuclease from the microbial clustered regularly interspaced short …

CRISPR-Cas systems enable microbial adaptive immunity and provide eukaryotic genome
editing tools. These tools employ a single effector enzyme of type II or V CRISPR to generate …

CRISPR-Cas-mediated genome editing relies on guide RNAs that direct site-specific DNA
cleavage facilitated by the Cas endonuclease. Here we report that chemical alterations to …

Abstract Streptococcus pyogenes (Spy) Cas9 has potential as a component of gene
therapeutics for incurable diseases. One of its limitations is its large size, which impedes its …

Type II CRISPR immune systems in bacteria use a dual RNA-guided DNA endonuclease,
Cas9, to cleave foreign DNA at specific sites. We show here that Cas9 assembles with …