F Macdonald, J R Sampson, C L Barratt, W Reik, and M M Hawkins

**Corrected Method and Results, Paragragh 1**

**NOTE: **Corrected
text appears in**BOLD**

We reviewed the notes of 149 BWS patients who had been referred to the
BWS Research Group at the Birmingham University Section of Medical Genetics
and/or the West Midlands Molecular Genetics Service (for uniparental disomy
analysis) and for whom detailed clinical information had been collected.
A history of assisted conception techniques was recorded for six cases
(4%) (table
1). To estimate whether his was likely to be a significantly increased
proportion, we compared the frequency of in vitro fertilisation IVF) and
ICSI births in the BWS cohort with that in the general population. The
first ART associated BWS case was born in 1989 and the most recent in 2002.
Data for the number of children born after ART are available for 1995,
1996, 1997,1998, 1999, and 2000 (http://www.hfea.gov.uk)
and during these years there was a total of 43 074 births after IVF or
ICSI to UK residents. The corresponding number of total births in the UK
was 4 320 482, so that 0.997% of births in the general population were
after IVF or ICSI. Based on these data, if the proportion of births after
IVF and ICSI in BWS patients and in the general population were similar,
we would have expected **1.4855 **of the 149 BWS patients studied to
have been born as a result of IVF or ICSI. To test the significance of
the observed and expected frequencies we used a Poisson approximation to
the binomial distribution and obtained a two tailed p value of **0.009**.
Thus, the observed frequency (n=6) of IVF and ICSI births in the BWS series
is significantly greater than the expected (**1.4855**), with an associated
95% confidence interval on the excess risk of 1.5, 8.8. It should be noted
that (1) although these calculations do not take account of maternal age,
there is no reported evidence that maternal age in BWS births differs from
that in the general population and (2) data on the frequency of IVF and
ICSI births are not available for years before 1995. Had such data been
available, then this would have very probably reduced the expected number
of BWS births after IVF or ICSI, as the birth rate in the general population
has been declining since 1989 and the annual number of ART births before
1995 would have been less than during the five years included in the comparison.
Therefore our comparison is likely to be conservative in relation to calendar
year.