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Biochemical genetics
Short report
TRAPγ-CDG shows asymmetric glycosylation and an effect on processing of proteins required in higher organisms
  1. Sabine Dittner-Moormann1,
  2. Charles Marques Lourenco2,
  3. Janine Reunert1,
  4. Ryuichi Nishinakamura3,
  5. Satomi S Tanaka3,
  6. Claudius Werner1,
  7. Volker Debus1,
  8. Klaus-Peter Zimmer4,
  9. Gabriele Wetzel5,
  10. Hassan Y Naim5,
  11. Yoshinao Wada6,
  12. Stephan Rust1,
  13. Thorsten Marquardt1
  1. 1 Department of Pediatrics, Universitätsklinikum Münster, Münster, Germany
  2. 2 Department of Medical Genetics, School of Medicine, Neurogenetics Unit, University, Sao Paulo, Sao Paulo, Brazil
  3. 3 Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Kumamoto, Japan
  4. 4 Department of Pediatrics, Universitätsklinikum Gießen und Marburg Standort Gießen, Giessen, Hessen, Germany
  5. 5 Department of Physiological Chemistry, University of Veterinary Medicine, Hannover, Germany, Hannover, Germany
  6. 6 Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi, Osaka, Japan
  1. Correspondence to Dr Thorsten Marquardt, Department of Pediatrics, Universitätsklinikum Münster, Munster, Germany; marquat{at}uni-muenster.de

Abstract

Newly synthesised glycoproteins enter the rough endoplasmic reticulum through a translocation pore. The translocon associated protein (TRAP) complex is located close to the pore. In a patient with a homozygous start codon variant in TRAPγ (SSR3), absence of TRAPγ causes disruption of the TRAP complex, impairs protein translocation into the endoplasmic reticulum and affects transport, for example, into the brush-border membrane. Furthermore, we observed an unbalanced non-occupancy of N-glycosylation sites. The major clinical features are intrauterine growth retardation, facial dysmorphism, congenital diarrhoea, failure to thrive, pulmonary disease and severe psychomotor disability.

  • CDG
  • TRAP
  • translocation
  • glycosylation

https://doi.org/10.1136/jmedgenet-2019-106279

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    Footnotes

    • SR and TM contributed equally.

    • Contributors TM planned the study and is responsible for the overall content. SD-M, CML, CW and VD conducted the study and did the clinical work-up. JR, SD-M and SR did the genetic analysis. RN and SST contributed samples from the knockout mouse. K-PZ, GW and HYN are responsible to the scientific investigation of the biopsies. YW performed mass spectrometry.

    • Funding The authors have not declared a specific grant for this research from any funding agency in the public, commercial or not-for-profit sectors.

    • Competing interests None declared.

    • Patient consent for publication Parental/guardian consent obtained.

    • Provenance and peer review Not commissioned; externally peer reviewed.