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Original article
Prevalence of BRCA1/2 germline mutations in 21 401 families with breast and ovarian cancer
  1. Karin Kast1,2,
  2. Kerstin Rhiem3,
  3. Barbara Wappenschmidt3,
  4. Eric Hahnen3,
  5. Jan Hauke3,
  6. Britta Bluemcke3,
  7. Verena Zarghooni3,
  8. Natalie Herold3,
  9. Nina Ditsch4,
  10. Marion Kiechle5,
  11. Michael Braun6,
  12. Christine Fischer7,
  13. Nicola Dikow7,
  14. Sarah Schott8,9,
  15. Nils Rahner10,
  16. Dieter Niederacher11,
  17. Tanja Fehm11,
  18. Andrea Gehrig12,
  19. Clemens Mueller-Reible12,
  20. Norbert Arnold13,
  21. Nicolai Maass13,
  22. Guntram Borck14,
  23. Nikolaus de Gregorio15,
  24. Caroline Scholz16,
  25. Bernd Auber16,
  26. Raymonda Varon-Manteeva17,
  27. Dorothee Speiser18,
  28. Judit Horvath19,
  29. Nadine Lichey19,
  30. Pauline Wimberger1,2,
  31. Sylvia Stark20,
  32. Ulrike Faust21,
  33. Bernhard H F Weber22,
  34. Gunter Emons23,
  35. Silke Zachariae24,
  36. Alfons Meindl5,
  37. Rita K Schmutzler3,
  38. Christoph Engel24
  39. on behalf of the German Consortium for Hereditary Breast and Ovarian Cancer (GC-HBOC)
  1. 1Department of Gynecology and Obstetrics, University Hospital Carl Gustav Carus, TU Dresden, Dresden, Germany
  2. 2German Cancer Consortium (DKTK) Dresden and German Cancer Research Center (DKFZ), Heidelberg, Germany
  3. 3Center for Hereditary Breast and Ovarian Cancer, Center for Integrated Oncology (CIO) and Center for Molecular Medicine Cologne (CMMC), Medical Faculty, University of Cologne and University Hospital Cologne, Cologne, Germany
  4. 4Department for Gynecology and Obstetrics, LMU Munich, Munich, Germany
  5. 5Department for Gynecology and Obstetrics, Technical University of Munich, Munich, Germany
  6. 6Breast Center, Department of Gynecology, Red Cross Hospital, Munich, Germany
  7. 7Institute of Human Genetics, Heidelberg University, Heidelberg, Germany
  8. 8Department of Gynecology and Obstetrics, Heidelberg University Hospital, Heidelberg, Germany
  9. 9German Cancer Consortium (DKTK), NCT Heidelberg and German Cancer Research Center (DKFZ), Heidelberg, Germany
  10. 10Medical Faculty, Institute of Human Genetics and Anthropology, Heinrich-Heine University, Düsseldorf, Germany
  11. 11Department of Gynecology and Obstetrics, Medical Faculty, Heinrich-Heine University, Düsseldorf, Germany
  12. 12Department of Human Genetics, University of Wuerzburg, Würzburg, Germany
  13. 13Department of Gynaecology and Obstetrics, University Hospital of Schleswig-Holstein, Campus Kiel, Christian-Albrechts University Kiel, Kiel, Germany
  14. 14Institute of Human Genetics, University of Ulm, Ulm, Germany
  15. 15Department of Gynecology and Obstetrics, University Hospital, Universität Ulm, Ulm, Germany
  16. 16Institute of Human Genetics, Hannover Medical School, Hannover, Germany
  17. 17Institute of Medical and Human Genetics, Charite-University Medical Center, Berlin, Germany
  18. 18Department of Gynecology, Charité University Medicine Berlin, Berlin, Germany
  19. 19Institute for Human Genetics, University of Muenster, Münster, Germany
  20. 20Department of Gynecology and Obstetrics, University of Leipzig, Leipzig, Germany
  21. 21Institute of Medical Genetics and Applied Genomics, University of Tuebingen, Tübingen, Germany
  22. 22Institute of Human Genetics, University of Regensburg, Regensburg, Germany
  23. 23Department of Obstetrics and Gynecology, University of Göttingen, Göttingen, Germany
  24. 24Institute for Medical Informatics, Statistics and Epidemiology, University of Leipzig, Leipzig, Germany
  1. Correspondence to Karin Kast, Department of Gynecology and Obstetrics, University Hospital Carl Gustav Carus, TU Dresden, Fetscherstraße 74, Dresden 01069, Germany; karin.kast{at}uniklinikum-dresden.de

Abstract

Purpose To characterise the prevalence of pathogenic germline mutations in BRCA1 and BRCA2 in families with breast cancer (BC) and ovarian cancer (OC) history.

Patients and methods Data from 21 401 families were gathered between 1996 and 2014 in a clinical setting in the German Consortium for Hereditary Breast and Ovarian Cancer, comprising full pedigrees with cancer status of all individual members at the time of first counselling, and BRCA1/2 mutation status of the index patient.

Results The overall BRCA1/2 mutation prevalence was 24.0% (95% CI 23.4% to 24.6%). Highest mutation frequencies were observed in families with at least two OCs (41.9%, 95% CI 36.1% to 48.0%) and families with at least one breast and one OC (41.6%, 95% CI 40.3% to 43.0%), followed by male BC with at least one female BC or OC (35.8%; 95% CI 32.2% to 39.6%). In families with a single case of early BC (<36 years), mutations were found in 13.7% (95% CI 11.9% to 15.7%). Postmenopausal unilateral or bilateral BC did not increase the probability of mutation detection. Occurrence of premenopausal BC and OC in the same woman led to higher mutation frequencies compared with the occurrence of these two cancers in different individuals (49.0%; 95% CI 41.0% to 57.0% vs 31.5%; 95% CI 28.0% to 35.2%).

Conclusions Our data provide guidance for healthcare professionals and decision-makers to identify individuals who should undergo genetic testing for hereditary breast and ovarian cancer. Moreover, it supports informed decision-making of counselees on the uptake of genetic testing.

  • Mutation prevalences
  • BRCA1
  • BRCA2
  • risk criteria
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Introduction

Women with pathogenic germline mutations in the BRCA1 and BRCA2 genes are at an increased lifetime risk for breast cancer (BC) and ovarian cancer (OC) compared with the general population.1 Identification of mutation carriers is an important prerequisite for targeted clinical management. The probability of finding a deleterious germline mutation in a woman affected with BC or OC depends on her familial cancer history in terms of type, number and ages of onset of these cancers.2–5 To date, the decision to perform mutation testing is mainly guided by the presence of a family cancer history, which is indicative for a BRCA mutation with a certain probability.6–8 Consequently, to define appropriate clinical selection criteria, precise knowledge on expected mutation frequencies for individual family histories is required. Since the discovery of the BRCA1 and BRCA2 genes, several studies have analysed the relationship between family history and BRCA mutation prevalence.9–14 In the largest study so far, Frank et al11 correlated familial disease histories of 10 000 consecutively enrolled individuals with mutation status, resulting in detailed tabulations of empiric mutation prevalences.

In 1996, the German Consortium for Hereditary Breast and Ovarian Cancer (GC-HBOC) had established a panel of clinical criteria for genetic testing of individuals in a clinical setting, based on familial BC and OC history.7 By the end of 2014, a total of 21 401 families suspected of having a deleterious BRCA mutation according to this panel of clinical criteria were enrolled into the central registry of GC-HBOC. Based on this data set, we aimed to comprehensively analyse the correlation of family history of BC and OC with BRCA mutation frequencies. We were particularly interested in the predictive value of the number of premenopausal versus postmenopausal BCs in the family, and the presence of premenopausal or postmenopausal bilateral breast cancer (bBC) versus unilateral cancer. With this analysis, we aim to provide guidance for counsellors, counselees and decision-makers on the offer and uptake of genetic testing.

Materials and methods

The GC-HBOC comprises 15 university centres. Using standardised clinical criteria (table 2), families with clustering or early onset of BC or OC are registered and tested for the presence of deleterious germline mutations in BRCA1 and BRCA2. Comprehensive data on familial cancer history, including a detailed pedigree, pathology reports and results of molecular testing, are documented in a central database using standardised electronic case report forms.

A total number of 21 401 families, who were registered from 1996 until 2014, were included in the present analysis. All families fulfilled the clinical inclusion criteria shown in table 2. Families who were ascertained through a known pathogenic mutation rather than by clinical criteria were not included. If possible, the family member with the most severe phenotype (defined as bBC, BC and OC, or earliest age of onset) was chosen as the index patient and was searched for BRCA1 and BRCA2 mutations. In case that no DNA from an affected family member could be obtained, mutation analysis was performed in unaffected individuals. BC included ductal carcinoma in situ. OC included cancers of the fallopian tube and primary peritoneal cancers irrespective of histopathological subtype and grading. BC cases with an age of onset of 50 years or earlier are hereafter referred to as premenopausal BC (BC50−). BC at the age from 51 years onwards are referred to as postmenopausal BC (BC51+). For bBC, the age of onset of the first cancer was considered. The present study did not include families with single cases of OC or single cases of male breast cancer (mBC) since these families were not part of the clinical inclusion criteria.

Mutation analysis

Mutation analysis was performed using direct sequencing or a pre-screening step followed by direct sequencing of suspect fragments. Pre-screening methods comprised mainly denaturing high-performance liquid chromatography and high-resolution melting.15 ,16 Before the year 1999, single-strand conformation polymorphism and protein truncation test were used. If no deleterious sequence alterations were found in these steps, an additional screening for large genomic alterations was performed using multiplex ligation-dependent probe amplification. In the present study, 88.4% of all BRCA1/2-negative index patients were searched for large genomic alterations. Mutations were classified according to the International Agency for Research on Cancer (IARC) system and considered pathogenic or likely pathogenic (class 4 or 5) based on literature evidence, multifactorial likelihood and functional analyses of the ENIGMA consortium that comprises genetic data of the GC-HBOC database.17–19

Statistical analysis

IBM SPSS 22 was used for statistical analysis. The 95% CIs for mutation frequencies were calculated using Wilson's score method. For comparison of mutation frequencies between groups, the χ2 test was used. p Values <0.05 were considered significant.

Results

Basic characteristics of the study population are summarised in table 1. Of 21 401 families, 3398 (15.9%) had a pathogenic mutation in BRCA1 and 1766 (8.3%) in BRCA2. Of these, 28 families had mutations in both genes, BRCA1 and BRCA2. The median number of family members documented in each pedigree was 25, regardless of BRCA mutation status. The mean age at cancer diagnosis was 44.4 years for BC and 49.8 years for OC in BRCA1-positive families compared with 47.5 and 55.6 years, in BRCA2-positive families, and 50.2 and 51.9 years in BRCA-negative families. The mean age at diagnosis of mBC in BRCA1-positive families was 56.9 years, which was younger than in BRCA2 mutation carriers (60.4 years) and equal to BRCA1/2-negative families (57.0 years).

Table 1

Family characteristics

The families were classified into seven mutually exclusive groups of aggregated familial cancer histories (table 2). These groups cover the inclusion criteria of the GC-HBOC established in 1996. The overall BRCA1/2 mutation prevalence was 24.0% (95% CI 23.4% to 24.6%). The highest mutation frequencies were seen in families with at least two OC (41.9%, 95% CI 36.1% to 48.0%) and families with at least one BC and one OC (41.6%, 95% CI 40.3% to 43.0%) followed by families with mBC and at least one additional female with BC or OC (35.8%, 95% CI 32.2% to 39.6%). BRCA1 mutations were more frequent in families with OC, whereas BRCA2 mutations were more frequent in families with mBC. The largest group comprises families with at least two cases of BC, at least one that was diagnosed before the age of 51 (n=12.996, 60.7% of all families). The lowest mutation frequencies (3.7%, 95% CI 2.5% to 5.3%) were observed in families with three or more cases of postmenopausal BC, but no occurrence of premenopausal BC, OC or male BC.

Table 2

Familial breast and ovarian cancer histories used as inclusion criteria for BRCA1/2 mutation testing in German Consortium for Hereditary Breast and Ovarian Cancer and observed mutation prevalences

To characterise mutation frequencies in more detail, the familial cancer histories were further refined. Table 3 shows the mutation prevalences in families, in which exclusively female BC was present (72.1% of all families). Group 1a comprises families with exclusive occurrence of unilateral cases of BC (53.1% of all families), whereas groups 1b (14.6%) and 1c (4.4%) include also cases of premenopausal and postmenopausal bilateral BC, respectively. These groups were further stratified by the number of women with premenopausal and postmenopausal BC. The highest mutation frequency was seen in families with at least three females with premenopausal BC, at least one of which was bilateral (50.7%, 95% CI 45.6% to 55.8%). Mutations were detected significantly more frequent in families with a single case of premenopausal bilateral BC than in families with two different women with premenopausal BC (22.7%, 95% CI 19.2% to 26.7% vs 17.5%, 95% CI 15.8% to 19.4%, p=0.012). In families with a single case of very early BC before the age of 36, mutations were found in 13.7% (95% CI 11.9% to 15.7%). In all subgroups, the occurrence of additional cases of postmenopausal BC did not considerably change mutation frequencies. In contrast, additional cases of premenopausal BC increased the mutation frequencies considerably.

Table 3

BRCA mutation prevalence in families with female breast cancer only

Table 4 lists families with OC only (group 2a, 1.2% of all families), families with BC and OC (group 2b, 23.7%) and families with occurrence of mBC (group 3, 3.0%). In families with one case of BC and one case of OC, mutation frequencies were considerably higher when the BC case was premenopausal (31.5% 95% CI 28.0% to 35.2% vs 19.2%, 95% CI 15.3% to 23.8%). Double primary premenopausal BC and OC in one individual was associated with a much higher mutation prevalence than the occurrence of premenopausal BC and OC in two different women (49.0%, 95% CI 41.0% to 57.0% vs 31.5%, 95% CI 28.0% to 35.2, p<0.001). However, there was no significant difference in mutation prevalence between double primary BC and OC (BCOC) and occurrence of BC and OC in two different women (BC/OC) when the BC was postmenopausal instead of premenopausal (20.3%, 95% CI 14.1% to 28.5% vs 19.2%, 95% CI 15.3% to 23.8, p=0.788).

Table 4

BRCA mutation prevalence in families with ovarian cancer (OC) only (group 2a), both breast cancer (BC) and OC (group 2b), and male breast cancer (mBC) (group 3)

In families with mBC (table 4, group 3) and an additional case of female BC, no significant difference in mutation frequencies could be detected depending on menopausal status of the BC (16.5%, 95% CI 10.4% to 25.1% vs 23.2%, 95% CI 16.0% to 32.5%, p=0.284). In families with mBC and additional cases of OC, mutation frequencies for BRCA1 and BRCA2 mutations were similar in contrast to families without additional OC cases.

Discussion

Based on a large sample of 21 401 families, the present study provides a detailed characterisation of BRCA1/2 mutation prevalences for defined patterns of familial BC and OC. The underlying data were collected over a period of almost 20 years (1996–2015) in a standardised way within a German multicentre consortium of interdisciplinary university centres specialised in providing healthcare for families with HBOC. All families suspected of having HBOC were selected for genetic testing according to a set of defined clinical criteria that were compulsory for all participating centres.

The German HBOC consortium has defined inclusion criteria for BRCA1/2 testing based on at least three generation pedigree analysis.6 Currently, GC-HBOC offers genetic testing to index patients if the expected BRCA mutation probability is ≥10% based on the individual family cancer history. In our study, the overall mutation prevalence was 24.0%. The decision threshold of 10% is exceeded in almost all subgroups of HBOC families including mBC. However, families with exclusive occurrence of three or more postmenopausal BC cases (3.2% of all families) were below the 10% threshold with a mutation of prevalence of only 3.7%. The latter finding is in line with the study of Frank et al,11 who reported a prevalence of 3.9%. Interestingly, the low prevalence observed in our study was independent of whether bilateral cases were present among the postmenopausal BCs in the family (3.1%) or not (3.8%). Moreover, mutation prevalences did not increase with the number of postmenopausal BC cases in the family. On the contrary, in a previous study we showed that an increasing number of females with BC diagnosed at an age of 60 or later was associated with a decreasing BRCA1/2 mutation frequency.20

In contrast to postmenopausal BC, mutation prevalence increased considerably with each additional case of premenopausal BC both in families with exclusive occurrence of BC and in families with BC and OC. This agrees well with the results of the study of Frank et al.11 In our study, mutation prevalence was even higher if at least one case of premenopausal BC was bilateral. In contrast, the presence of postmenopausal bilateral BC did not increase the chance to detect a deleterious mutation. This observation is in line with a previous study of Gershoni-Baruch et al,21 who suggested that bBC per se is not reflective of genetic predisposition, unless associated with early age of onset. However, other studies came to the conclusion that mutation frequencies are similar if two cancers (bBC or BCOC) occurred in one individual compared with two separate individuals.10 ,22–24 In our study, mutations were detected significantly more often in families with a single case of premenopausal bilateral BC than in families with two independent premenopausal BC (22.7% vs 17.5%, p=0.012).

In accordance with other studies, the highest mutation rates were observed in families with BC and OC.4 ,9 ,11 ,12 As observed in families with exclusive occurrence of BC, mutation prevalence was considerably higher if BC cases were premenopausal. Moreover, mutation prevalence was significantly higher in individuals with double primaries of ovarian and premenopausal BC than if these two cancers occurred in different women.

Our study comprised 642 families with mBC. Due to the inclusion criteria, these families had at least one additional case of female BC or OC. As described in previous studies, BRCA2 mutations were found more frequently than BRCA1 mutations in families with mBC but without occurrence of OC. In cases where additional OC cases were present in these families, a similar prevalence of mutations was found in BRCA1 and BRCA2. The additional presence of a premenopausal versus postmenopausal BC increased the mutation prevalence from 16.5% to 23.2%, although this difference was not significant due to low sample sizes in these groups.

The present study revealed a mutation prevalence of 13.7% (9.2%, 95% CI 7.7% to 10.9% for BRCA1 and 4.5%, 95% CI 3.5% to 5.8% for BRCA2) for individuals with early unilateral BC before the age of 36 years without further cancer cases in their family. This is in line with results from two other studies.25 ,26 However, there is evidence in the literature that the prevalence of BRCA1 and BRCA2 mutations is high in women with triple-negative breast cancer (TNBC) and that BRCA1/2 mutations are not restricted to young women or patients with a positive family history.27 Thus, single cases of TNBC might be considered in BRCA1/2 genetic testing guidelines with an extended age of onset that requires further exploration to confirm a potential cut-off at the age of 60 years as suggested by the data of Couch et al.27

Comparison of our data to empiric mutation frequencies of other groups is difficult due to varying inclusion criteria. For example, in high-risk HBOC families of Czech ancestry an overall detection rate of 29% was reported compared with 24.0% by us.12 That work was restricted to first-degree and second-degree relatives in the maternal line and to third-degree relatives in the case of paternal transmission. In the work of Frank et al,11 the overall detection frequency was 15.7% in non-Ashkenazi individuals, but postmenopausal BC was not taken into account.

Some limitations have to be mentioned. First, in our study only aggregated familial cancer histories were correlated with mutation prevalence, that is, the pedigree size and family structure was not considered. Mutation prediction algorithms such as BOADICEA, BRCAPRO or IBIS, which use the pedigree structure and an underlying genetic model of inheritance, might have a better predictive performance.28–31 Second, it is known that BRCA1/2-associated OCs are characterised by a high grade (G2/3) serous histology.32–34 In our study, we did not restrict the analysis to OC with specific pathological features since pathology information was not available for all OC. Thus, we cannot exclude that a restriction to this specific type of OC would have led to larger mutation frequencies.

In summary, we provide a detailed overview on empiric BRCA1/2 mutation frequencies for at-risk individuals with different familial cancer histories. The number of postmenopausal BC failed to show a systematic correlation with mutation frequency. Bilateral BC or premenopausal BC and OC in one affected family member conferred a higher mutation prevalence than two primaries in separate individuals. Our analysis provides a simple means to get an overview about expected mutation probabilities during clinical and genetic counselling before mutation testing is considered for both counsellors and counselees. Moreover, our results provide guidance for healthcare professionals and decision-makers to define consistent clinical criteria for decision-making to undergo genetic testing for individuals with suspected HBOC.

Acknowledgments

The authors thank all the participating women and the German Cancer Aid for supporting GC-HBOC.

References

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Footnotes

  • Contributors KK: conception of the work, acquisition and interpretation of data, writing of the manuscript, final approval of the version to be published. AM and RKS: conception of the work, acquisition and interpretation of data, critical revision of the manuscript for important intellectual content, final approval of the version to be published. CE: conception of the work, analysis and interpretation of data, writing of the manuscript, final approval of the version to be published. All other authors: acquisition of data, critical revision of the manuscript for important intellectual content, final approval of publication. All authors agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

  • Funding Deutsche Krebshilfe (German Cancer Aid), grant no. 110837.

  • Competing interests None declared.

  • Patient consent Obtained.

  • Ethics approval The study has been approved by the Institutional Review Boards of each participating centre.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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