Background: Oxidative stress has been recently suggested to play a part in the development of osteoporosis. Catalase is a major antioxidant enzyme that detoxifies hydrogen peroxide by converting it into water and oxygen, thereby preventing cellular injury by oxidative stress.
Aims: To examine the associations between the catalase gene (CAT) polymorphisms and bone mineral density (BMD) and bone turnover markers in postmenopausal Korean women.
Methods: All exons, their boundaries and the promoter region (approximately 1.5 kb) were directly sequenced in 24 individuals. Among 18 variants identified by a direct sequence method, four polymorphisms were selected and genotyped in all study participants (n = 560). BMD at the lumbar spine and proximal femur was measured using dual-energy x ray absorptiometry. Serum osteocalcin concentrations and bone-specific alkaline phosphatase activity were determined by an immunoradiometric assay and an immunoassay, respectively.
Results: The mean (standard deviation) age of the participants was 59.4 (7.2) years. Multivariate analysis showed an association of the +22348C→T polymorphism with BMD at the lumbar spine (p = 0.01 in the dominant model) and at femur neck (p = 0.05 in the dominant model), and with serum osteocalcin level (p = 0.008 in the dominant model). Haplotype analyses showed that HT4 (−20T, +144C, +22348T, +33078A) was significantly associated with higher BMD at various sites (p<0.001–0.03) and with lower serum osteocalcin levels (p = 0.01 in the codominant model).
Conclusions: These findings indicate that the +22348C→T polymorphism and HT4 of CAT may be useful genetic markers for bone metabolism.
- BMD, bone mineral density
- CAT, catalase gene
- ROS, reactive oxygen species
- SNP, single-nucleotide polymorphism
- YSM, years since menopause
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↵* These authors contributed equally to this work.
Funding: This work was supported by a grant from the Korea Health 21 R & D Project, Ministry of Health and Welfare, Republic of Korea (Project 01-PJ3-PG6-01GN111-0002).
Competing interests: None.
Ethical approval: This study was approved by the institutional review board of the Asan Medical Center, and written informed consent was obtained from each participant.