Article Text

Download PDFPDF
Reverse chromosome painting: a method for the rapid analysis of aberrant chromosomes in clinical cytogenetics.
  1. N P Carter,
  2. M A Ferguson-Smith,
  3. M T Perryman,
  4. H Telenius,
  5. A H Pelmear,
  6. M A Leversha,
  7. M T Glancy,
  8. S L Wood,
  9. K Cook,
  10. H M Dyson
  1. Department of Pathology, University of Cambridge.


    We describe a method, termed reverse chromosome painting, which allows the rapid analysis of the content and breakpoints of aberrant chromosomes. The method involves the sorting of small numbers of the aberrant chromosome from short term blood culture preparations or cell lines by using bivariate flow karyotype analysis. The sorted chromosomes are amplified and biotin labelled enzymatically using a degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR), the product annealed to metaphase spreads from normal subjects, and hybridisation detected using fluorescence in situ hybridisation (FISH). We show the usefulness of this method for routine clinical cytogenetics by the analysis of cases involving an insertion, a deletion, a translocation, and two cases of a chromosome with additional material of unknown origin. The method has particular application for the rapid resolution of the origin of de novo unbalanced chromosome duplications.

    Statistics from

    Request Permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.