Abstract
Spinal muscular atrophy (SMA) is a common motor neuron degenerative disease that results from reduced levels of, or mutations in, the Survival of Motor Neurons (SMN) protein. SMN is found in the cytoplasm and the nucleus where it is concentrated in gems. SMN interacts with spliceosomal snRNP proteins and is critical for snRNP assembly in the cytoplasm. We show that a dominant-negative mutant SMN (SMNdeltaN27) causes a dramatic reorganization of snRNPs in the nucleus. Furthermore, SMNdeltaN27 inhibits pre-mRNA splicing in vitro, while wild-type SMN stimulates splicing. SMN mutants found in SMA patients cannot stimulate splicing. These findings demonstrate that SMN plays a crucial role in the generation of the pre-mRNA splicing machinery and thus in mRNA biogenesis, and they link the function of SMN in this pathway to SMA.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Autoantigens / metabolism
-
Cyclic AMP Response Element-Binding Protein
-
HeLa Cells
-
Humans
-
Microscopy, Fluorescence
-
Muscular Atrophy, Spinal / genetics*
-
Nerve Tissue Proteins / genetics
-
Nerve Tissue Proteins / physiology*
-
Nuclear Proteins / metabolism
-
Open Reading Frames
-
Organelles / metabolism
-
Organelles / ultrastructure
-
Phosphoproteins / metabolism
-
RNA Precursors / genetics*
-
RNA Splicing*
-
RNA-Binding Proteins
-
Ribonucleoproteins, Small Nuclear / metabolism
-
SMN Complex Proteins
-
Spliceosomes / metabolism
Substances
-
Autoantigens
-
Cyclic AMP Response Element-Binding Protein
-
Nerve Tissue Proteins
-
Nuclear Proteins
-
Phosphoproteins
-
RNA Precursors
-
RNA-Binding Proteins
-
Ribonucleoproteins, Small Nuclear
-
SMN Complex Proteins
-
p80-coilin