Abstract
Epididymal fluid from rats rendered infertile by oral administration of ornidazole contains a protein CAP1 (contraception-associated protein 1) that is absent from epididymal fluid, but present on epididymal sperm, from fertile vehicle-treated rats. The gene for CAP1 has been isolated from a rat testis cDNA library and its expression investigated in different tissues. The deduced protein sequence of CAP1 contains 189 amino-acid residues and database searches revealed a high degree of homology (83-95%) with human and mouse DJ-1 at the nucleotide and amino acid levels. Northern blot hybridisation from different rat tissues indicated that CAP1 is encoded by a 1.6-kilobase RNA transcript and seems to be ubiquitiously expressed in the rat with a high level of expression in the testis.
Copyright 1998 Academic Press.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular
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DNA, Complementary
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Epididymis / physiology*
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Fertility
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Fertilization*
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Gene Library
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Humans
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Infertility, Male / chemically induced
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Infertility, Male / metabolism
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Intracellular Signaling Peptides and Proteins
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Male
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Mice
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Molecular Sequence Data
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Oncogene Proteins / chemistry
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Organ Specificity
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Ornidazole / pharmacology
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Protein Biosynthesis
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Protein Deglycase DJ-1
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Proteins / chemistry
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Proteins / genetics*
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Rats
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Rats, Sprague-Dawley
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Sequence Alignment
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Spermatozoa / metabolism*
Substances
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DNA, Complementary
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Intracellular Signaling Peptides and Proteins
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Oncogene Proteins
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Proteins
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Recombinant Proteins
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contraception associated protein 1
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Ornidazole
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PARK7 protein, human
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Protein Deglycase DJ-1