Undermethylation of specific LINE-1 sequences in human cells producing a LINE-1-encoded protein

R E Thayer; M F Singer; T G Fanning

doi:10.1016/0378-1119(93)90651-i

Undermethylation of specific LINE-1 sequences in human cells producing a LINE-1-encoded protein

Gene. 1993 Nov 15;133(2):273-7. doi: 10.1016/0378-1119(93)90651-i.

Authors

R E Thayer¹, M F Singer, T G Fanning

Affiliation

¹ Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.

PMID: 7693554
DOI: 10.1016/0378-1119(93)90651-i

Abstract

Nucleotide sequences near the 5' ends of some long interspersed elements-1 (LINE-1) from Homo sapiens (L1Hs) are undermethylated in cell lines which produce a L1Hs-encoded protein. In contrast, these sequences are methylated in cell lines with little or no detectable L1Hs expression. The fact that the 5' end of L1Hs is differentially methylated in cells exhibiting different levels of L1Hs expression suggests that the methylation state of this region plays a role in L1Hs expression.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Blotting, Southern
Blotting, Western
Cell Line
Cytosine / metabolism
DNA Transposable Elements*
Deoxyribonuclease HpaII
Deoxyribonucleases, Type II Site-Specific / metabolism
Gene Expression Regulation
Humans
Methylation
Polymerase Chain Reaction
Protein Biosynthesis
Proteins / chemistry
Proteins / genetics

Substances

DNA Transposable Elements
Proteins
Cytosine
Deoxyribonuclease HpaII
Deoxyribonucleases, Type II Site-Specific