Abstract
Permanent lymphoblastoid cell lines are of great practical value in human clinical and experimental genetics. A detailed protocol for routine use is given for the establishment of lymphoblastoid lines from peripheral blood using Epstein-Barr virus and the immunosuppressivum Cyclosporin A. In addition, the biologic basis of this transformation system is briefly summarized.
MeSH terms
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Antigens, Viral / analysis
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B-Lymphocytes / cytology*
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B-Lymphocytes / microbiology
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B-Lymphocytes / ultrastructure
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Cell Division / drug effects
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Cell Line*
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Cell Transformation, Viral*
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Chromosome Aberrations*
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Cyclosporins / pharmacology
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Epstein-Barr Virus Nuclear Antigens
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Ethidium / pharmacology
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Herpesvirus 4, Human / immunology
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Humans
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Karyotyping
Substances
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Antigens, Viral
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Cyclosporins
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Epstein-Barr Virus Nuclear Antigens
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Ethidium