Seemingly neutral polymorphic variants may confer immunity to splicing-inactivating mutations: a synonymous SNP in exon 5 of MCAD protects from deleterious mutations in a flanking exonic splicing enhancer

Am J Hum Genet. 2007 Mar;80(3):416-32. doi: 10.1086/511992. Epub 2007 Jan 18.

Abstract

The idea that point mutations in exons may affect splicing is intriguing and adds an additional layer of complexity when evaluating their possible effects. Even in the best-studied examples, the molecular mechanisms are not fully understood. Here, we use patient cells, model minigenes, and in vitro assays to show that a missense mutation in exon 5 of the medium-chain acyl-CoA dehydrogenase (MCAD) gene primarily causes exon skipping by inactivating a crucial exonic splicing enhancer (ESE), thus leading to loss of a functional protein and to MCAD deficiency. This ESE functions by antagonizing a juxtaposed exonic splicing silencer (ESS) and is necessary to define a suboptimal 3' splice site. Remarkably, a synonymous polymorphic variation in MCAD exon 5 inactivates the ESS, and, although this has no effect on splicing by itself, it makes splicing immune to deleterious mutations in the ESE. Furthermore, the region of MCAD exon 5 that harbors these elements is nearly identical to the exon 7 region of the survival of motor neuron (SMN) genes that contains the deleterious silent mutation in SMN2, indicating a very similar and finely tuned interplay between regulatory elements in these two genes. Our findings illustrate a mechanism for dramatic context-dependent effects of single-nucleotide polymorphisms on gene-expression regulation and show that it is essential that potential deleterious effects of mutations on splicing be evaluated in the context of the relevant haplotype.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyl-CoA Dehydrogenase / genetics*
  • Cyclic AMP Response Element-Binding Protein / genetics
  • Cyclic AMP Response Element-Binding Protein / metabolism
  • DNA Primers / genetics
  • Enhancer Elements, Genetic / genetics*
  • Exons / genetics*
  • Female
  • Genes, BRCA1 / physiology
  • Humans
  • Immunity
  • Infant
  • Infant, Newborn
  • Lipid Metabolism, Inborn Errors / genetics
  • Lipid Metabolism, Inborn Errors / immunology*
  • Molecular Sequence Data
  • Muscular Atrophy, Spinal / genetics
  • Muscular Atrophy, Spinal / immunology
  • Mutation, Missense / genetics
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism
  • Polymorphism, Single Nucleotide*
  • RNA Splicing / genetics*
  • RNA Stability / genetics
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism
  • SMN Complex Proteins
  • Sequence Homology, Nucleic Acid
  • Silencer Elements, Transcriptional / genetics*
  • Survival of Motor Neuron 2 Protein
  • Transcription, Genetic

Substances

  • Cyclic AMP Response Element-Binding Protein
  • DNA Primers
  • Nerve Tissue Proteins
  • RNA-Binding Proteins
  • SMN Complex Proteins
  • SMN2 protein, human
  • Survival of Motor Neuron 2 Protein
  • Acyl-CoA Dehydrogenase

Associated data

  • GENBANK/AL592082
  • GENBANK/L78833