The oligomycin axis of mitochondrial ATP synthase: OSCP and the proton channel

R J Devenish; M Prescott; G M Boyle; P Nagley

doi:10.1023/a:1005621125812

The oligomycin axis of mitochondrial ATP synthase: OSCP and the proton channel

J Bioenerg Biomembr. 2000 Oct;32(5):507-15. doi: 10.1023/a:1005621125812.

Authors

R J Devenish¹, M Prescott, G M Boyle, P Nagley

Affiliation

¹ Department of Biochemistry and Molecular Biology, P.O. Box 13D, Monash University, Victoria 3800, Australia.

PMID: 15254386
DOI: 10.1023/a:1005621125812

Abstract

Oligomycin has long been known as an inhibitor of mitochondrial ATP synthase, putatively binding the F(o) subunits 9 and 6 that contribute to proton channel function of the complex. As its name implies, OSCP is the oligomycin sensitivity-conferring protein necessary for the intact enzyme complex to display sensitivity to oligomycin. Recent advances concerning the structure and mechanism of mitochondrial ATP synthase have led to OSCP now being considered a component of the peripheral stator stalk rather than a central stalk component. How OSCP confers oligomycin sensitivity on the enzyme is unknown, but probably reflects important protein-protein interactions made within the assembled complex and transmitted down the stator stalk, thereby influencing proton channel function. We review here our studies directed toward establishing the stoichiometry, assembly, and function of OSCP in the context of knowledge of the organization of the stator stalk and the proton channel.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Adenosine Triphosphatases / chemistry
Adenosine Triphosphatases / genetics
Adenosine Triphosphatases / metabolism*
Animals
Binding Sites
Carrier Proteins / chemistry
Carrier Proteins / genetics
Carrier Proteins / metabolism*
Membrane Proteins / chemistry
Membrane Proteins / genetics
Membrane Proteins / metabolism*
Mitochondrial Proton-Translocating ATPases / antagonists & inhibitors*
Mitochondrial Proton-Translocating ATPases / chemistry
Mitochondrial Proton-Translocating ATPases / metabolism
Models, Molecular
Multiprotein Complexes
Oligomycins / pharmacology*
Protein Subunits
Rats
Recombinant Proteins / chemistry
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Saccharomyces cerevisiae / enzymology

Substances

Carrier Proteins
Membrane Proteins
Multiprotein Complexes
Oligomycins
Protein Subunits
Recombinant Proteins
Adenosine Triphosphatases
Mitochondrial Proton-Translocating ATPases
oligomycin sensitivity-conferring protein