Specific metaphase and interphase detection of the breakpoint region in 8q24 of Burkitt lymphoma cells by triple-color fluorescence in situ hybridization

T Ried; C Lengauer; T Cremer; J Wiegant; A K Raap; M van der Ploeg; P Groitl; M Lipp

doi:10.1002/gcc.2870040109

Specific metaphase and interphase detection of the breakpoint region in 8q24 of Burkitt lymphoma cells by triple-color fluorescence in situ hybridization

Genes Chromosomes Cancer. 1992 Jan;4(1):69-74. doi: 10.1002/gcc.2870040109.

Authors

T Ried¹, C Lengauer, T Cremer, J Wiegant, A K Raap, M van der Ploeg, P Groitl, M Lipp

Affiliation

¹ Institute of Human Genetics and Anthropology, University of Heidelberg, Federal Republic of Germany.

PMID: 1377011
DOI: 10.1002/gcc.2870040109

Abstract

Triple fluorescence in situ hybridization with a plasmid DNA library from sorted human chromosomes 8 in combination with bacteriophage clones flanking the breakpoint in 8q24 of the Burkitt lymphoma cell line J1 was used for the specific delineation of this breakpoint in individual tumor cells. With this approach, tumor-specific breakpoints in translocation chromosomes can be detected at all stages of the cell cycle with high specificity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Burkitt Lymphoma / genetics*
Burkitt Lymphoma / pathology
Chromosome Banding
Chromosomes, Human, Pair 2 / ultrastructure
Chromosomes, Human, Pair 8 / ultrastructure*
DNA Probes
Fluorescent Dyes
Humans
Interphase
Male
Metaphase
Microscopy, Fluorescence
Nucleic Acid Hybridization*
Oncogenes
Plasmids
Translocation, Genetic*
Tumor Cells, Cultured / ultrastructure

Substances

DNA Probes
Fluorescent Dyes