Epigenetic change in IGF2R is associated with fetal overgrowth after sheep embryo culture

L E Young; K Fernandes; T G McEvoy; S C Butterwith; C G Gutierrez; C Carolan; P J Broadbent; J J Robinson; I Wilmut; K D Sinclair

doi:10.1038/84769

Epigenetic change in IGF2R is associated with fetal overgrowth after sheep embryo culture

Nat Genet. 2001 Feb;27(2):153-4. doi: 10.1038/84769.

Authors

L E Young¹, K Fernandes, T G McEvoy, S C Butterwith, C G Gutierrez, C Carolan, P J Broadbent, J J Robinson, I Wilmut, K D Sinclair

Affiliation

¹ Roslin Institute (Edinburgh), Roslin, Midlothian, Scotland, UK. Lorraine.Young@bbsrc.ac.uk

PMID: 11175780
DOI: 10.1038/84769

Abstract

Manipulation or non-physiological embryo culture environments can lead to defective fetal programming in livestock. Our demonstration of reduced fetal methylation and expression of ovine IGF2R suggests pre-implantation embryo procedures may be vulnerable to epigenetic alterations in imprinted genes. This highlights the potential benefits of epigenetic diagnostic screening in developing embryo procedures.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Abnormalities, Multiple / veterinary
Animals
Blastocyst / physiology*
Cloning, Organism / veterinary*
Congenital Abnormalities / veterinary
DNA Methylation
Embryonic and Fetal Development
Female
Genetic Testing
Genomic Imprinting
Pregnancy
Receptor, IGF Type 2 / genetics*
Sheep*

Substances

Receptor, IGF Type 2