Abstract
Using a differential display technique, we identified two genes that are down-regulated in human gastric cancer tissue as compared to normal gastric mucosa. The down-regulated expression of these genes in gastric cancer tissue was confirmed by northern blotting analysis and RT-PCR. One, CA11, was a novel gene expressed predominantly in the stomach and was depleted in all of the gastric cancer cell lines examined. The other gene, GC36, was homologous to the digestive tract-specific calpain gene, nCL-4. The expression of both GC36 and nCL-4 was suppressed or depleted in gastric cancer cell lines of differentiated and poorly differentiated types. This is the first report of genes, the expression of which is down-regulated with considerable frequency in gastric cancer.
MeSH terms
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Alternative Splicing
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Amino Acid Sequence
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Base Sequence
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Blotting, Northern
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Calpain / genetics*
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Calpain / metabolism
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Chromosomes, Human, Pair 1 / genetics
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Chromosomes, Human, Pair 2 / genetics
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DNA, Complementary / isolation & purification
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DNA, Complementary / metabolism
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DNA, Neoplasm / isolation & purification
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DNA, Neoplasm / metabolism
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Down-Regulation
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Gastric Mucosa / metabolism
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Gene Expression Regulation, Neoplastic
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Humans
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Isoenzymes / genetics
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Molecular Sequence Data
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Neoplasm Proteins / genetics*
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Neoplasm Proteins / metabolism
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RNA, Messenger / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Stomach Neoplasms / genetics*
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Stomach Neoplasms / metabolism
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Tumor Cells, Cultured
Substances
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DNA, Complementary
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DNA, Neoplasm
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Isoenzymes
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Neoplasm Proteins
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RNA, Messenger
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Calpain
Associated data
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GENBANK/AB038463
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GENBANK/AB039886