Rapid CommunicationsWNT1 inducible signaling pathway protein 3, WISP-3, a novel target gene in colorectal carcinomas with microsatellite instability☆,☆☆,★
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Patients and tumor samples
The present study included 275 primary tumors from 273 Norwegian colorectal cancer patients: 252 patients from a consecutive series and 21 selected patients with tumors located in the right colon. From each of 24 primary tumors, 2 or more biopsy specimens were collected and totally 303 lesions were analyzed. Three patients satisfied the Amsterdam criteria for hereditary nonpolyposis colorectal cancer.22 All tumors were classified according to the World Health Organization recommendations.23 One
Results
MSI at 1 or more loci was found in 70 of 275 (25%) of the carcinomas. Forty-three biopsy specimens from 36 tumors were MSI-H, 34 biopsy specimens from 33 tumors were MSI-L, and 2 biopsy specimens from 1 tumor were MSI-H and MSI-L, respectively.
Among the 24 primary tumors with 2 or more biopsies performed, 7 contained biopsy specimens with MSI. Intratumor heterogeneity was observed in 2 of the 7 tumors. Both biopsy specimens from 1 of the tumors were MSI-H, but only 1 of them showed changes in
Discussion
The frequency of MSI-H tumors (13%) reported in the present study is within the range of previous studies.6, 30 The present study confirmed that alterations in BAT-25 and BAT-26 identify the tumors prone for mutations in downstream target genes.31
MSI intratumor heterogeneity was seen in 2 of the primary tumors with more than 1 biopsy specimen analyzed. This result is different from a previous report that observed MSI throughout all of the 4 investigated areas of 6 analyzed primary tumors.32
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Cited by (0)
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Supported by grants from the Norwegian Cancer Society (NCS).
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Address requests for reprints to: Ragnhild A. Lothe, M.Sc., Ph.D., Department of Genetics, Institute for Cancer Research, The Norwegian Radium Hospital, 0310 Oslo, Norway. e-mail: [email protected]; fax: (47) 22934440.
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Dr. Thorstensen is a research fellow of the NCS.