Abstract
T cell non-Hodgkin's lymphomas are a heterogeneous group of lymphomas with poor prognosis, and whose genetic alterations are not well understood. Comparative genomic hybridization (CGH) is a technique that allows the identification of DNA imbalances without cytogenetic studies. We have studied 37 samples from 29 T cell non-Hodgkin's lymphomas (25 peripheral and four lymphoblastic lymphomas) by CGH in order to detect DNA sequence copy number changes of putative importance in the biology and prognosis of these neoplasms. We detected abnormal CGH profiles in 16/27 (59%) of samples at diagnosis, a ratio that increased to 66% (23/37) when we included the relapsed samples. The most common recurrent changes were gains related to the X chromosome, either the whole chromosome or partially the Xq26–27 bands (19%). Other recurrent changes included gains of bands 9q34, gains of chromosomes 17, 19, and 20, and complete or partial deletions of chromosome 13 (10%). Cancer-related genes located at Xq26–28 region were analyzed by Southern blot and fluorescence in situ hybridization (FISH). Low level amplification of some of these genes was detected by this technique confirming the results obtained by CGH in this region. The detection of abnormal CGH profiles in these T cell lymphomas could have clinical implications. Patients with abnormal CGH profiles showed significant associations with advanced stage of disease, overexpression of P53, and higher proliferative index.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Koeppen H, Vardinam J . New entities, issues and controversies in the classification of malignant lymphoma Semin Oncol 1998 25: 421–434
Sandberg AA . The Chromosomes in Human Cancer and Leukemia Elsevier: New York 1990
Schlegelberger B, Zhang Y, Weber-Matthiesen K, Grote W . Detection of aberrant clones in nearly all cases of angioimmunoblastic lymphadenopathy with dysproteinemia-type T-cell lymphoma by combined interphase and metaphase cytogenetics Blood 1994 84: 2640–2648
Schlegelberger B, Himmler A, Bartles H, Kuse R, Sterry W, Grote W . Recurrent chromosome abnormalities in peripheral T-cell lymphomas Cancer Genet Cytogenet 1994 78: 15–22
Schlegelberger B, Feller AC . Classification of peripheral T-cell lymphomas: cytogenetic findings support the updated Kiel classification Leuk Lymphoma 1996 20: 411–416
Weidmann E . Hepatosplenic T-cell lymphoma. A review on 45 cases since the first report describing the disease as a distinct lymphoma entity in 1990 Leukemia 2000 14: 991–997
Harris NL, Jaffe ES, Diebold J, Flandrin G, Muller-Hermelink HK, Vardiman J, Lister TA, Bloomfield CD . The World Health Organization classification of neoplastic diseases of the haematopoietic and lymphoid tissues: report of the Clinical Advisory Committee meeting-Airlie House, Virginia, November 1997 J Clin Oncol 1999 17: 3835–3849
Stansfeld AG, Diebold J, Kapanci Y, Kelényi G, Lennert K, Mioduszewska O, Noel H, Rilke F, Sundstrom C, Van Unnik JAM, Wright DH . Updated Kiel classiffication for Lymphomas (letter) Lancet 1988 1: 292–293
Rivas C, Echezarreta G, Garcia R, Santos M, Santon A, Robledo M, Benitez J, Oliva H, Delsol G . A multiparametric study of malignant lymphoma of mucosa associated lymphoid tissue (Malt) Leuk Lymphoma 1992 8: 87–96
Trainor JK, Brisco MJ, Wan JH, Nech S, Grist S, Morley AA . Gene rearrangement in B- and T-lymphoproliferative disease detected by the polimerase chain reaction Blood 1991 78: 192–196
Kallionemi A, Kallioniemi OP, Sudar D, Rutovitz D, Gray JW, Waldman F, Pinkel D . Comparative genomic hybridization for molecular cytogenetic analysis of solid tumours Science 1992 258: 818–821
Kallionemi OP, Kallioniemi A, Piper J, Isola J, Waldman F, Gray JW, Pinkel D . Optimizing comparative genomic hybridization for analysis of DNA sequence copy number changes in solid tumours Genes Chromosom Cancer 1994 10: 231–243
Schlegelberger B, Zwingers T, Hohenadel K, Henne-Bruns D, Schmitz N, Haferlach T, Tirier C, Bartels H, Sonnen R, Kuse R et al. Significance of cytogenetic findings for the clinical outcome in patients with T-cell lymphoma of angioimmunoblastic lymphadenopathy type J Clin Oncol 1996 14: 593–599
Dierlamm J, Rosenberg C, Stul M, Pittaluga S, Wlodarska I, Michaux L, Dehaen M, Vehoef G, Thomas J, de Kelver W, Bakker-Schut T, Cassiman JJ, Raap AK, De Wolf-Peeters C, Van den Berghe H, Hagemeijer A . Characteristic pattern of chromosome gains and losses in marginal zone B cell lymphoma detected by comparative genomic hybridization Leukemia 1997 11: 747–758
Avet-Loiseau H, Vigier M, Moreau A, Mellerin MP, Gaillard F, Harousseau JL, Bataille R, Milpied N . Comparative genomic hybridization detects genomic abnormalities in 80% of follicular lymphomas Br J Haematol 1997 97: 119–122
Monni O, Joensuu H, Franssila K, Knuutila S . DNA copy number changes in diffuse large B-cell lymphoma. Comparative Genomic Hybridization study Blood 1996 12: 5269–5278
Knuutila S, Björkqvist AM, Autio K, Tarkkanen M, Wolf M, Monni O, Szymanska J, Larramendy ML, Tapper J, Pere H, EI-Rifai W, Hemmer S, Wasenius VM, Vidgren V, Zhu Y . DNA copy number amplifications in human neoplasms Am J Pathol 1998 152: 1107–1126
Cigudosa JC, Rao PH, Calasanz MJ, Odero MD, Michaeli J, Jhanwar SC, Chaganti RSK . Characterization of nonrandom chromosomal gains and losses in multiple myeloma by comparative genomic hybridization Blood 1998 8: 3007–3010
Avet-Loiseau H, Andree-Ashley LE, Moore D 2nd, Mellerin MP, Feusner J, Bataille R, Pallavicini MG . Molecular cytogenetic abnormalities in multiple myeloma and plasma cell leukemia measured using comparative genomic hybridization Genes Chromosom Cancer 1997 19: 124–133
Barth T, Döhner H, Werner CA, Stilgenbauer S, Schlotter M, Pawlita M, Lichter P, Möller P, Bentz M . Characteristic pattern of chromosomal gains and losses in primary large B-cell lymphomas of the gastrointestinal tract Blood 1998 91: 4321–4330
Rao PH, Houldsworth J, Dyomina K, Parsa NZ, Cigudosa JC, Louie DC, Popplewell L, Offit K, Jhanwar SC, Chaganti RS . Chromosomal and gene amplification in diffuse large B-cell lymphoma Blood 1998 92: 234–240
Stern MH, Soulier J, Rozenzwajg M, Nakahara K, Canki-Klain N, Aurias A, Sigaux F, Kirsch IR . MTCP1: a novel gene on the human chromosome Xq28 translocated to the T cell receptor alpha/delta locus in mature T cell proliferations Oncogene 1993 8: 2475–2483
Acknowledgements
We thank Alicia Barroso and Jesus Gallego for their technical assistance. This work has been partially supported by CAM 99/086/0008, and CAM 08.1/0020/00 proyects. A Martinez-Ramirez is a fellow of the Instituto de Salud Carlos III.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Renedo, M., Martinez-Delgado, B., Arranz, E. et al. Chromosomal changes pattern and gene amplification in T cell non-Hodgkin's lymphomas. Leukemia 15, 1627–1632 (2001). https://doi.org/10.1038/sj.leu.2402248
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.leu.2402248
Keywords
This article is cited by
-
Pathogenetic and diagnostic significance of microRNA deregulation in peripheral T-cell lymphoma not otherwise specified
Blood Cancer Journal (2014)
-
High-resolution analysis of chromosome copy number alterations in angioimmunoblastic T-cell lymphoma and peripheral T-cell lymphoma, unspecified, with single nucleotide polymorphism-typing microarrays
Leukemia (2008)
-
Novel t(5;9)(q33;q22) fuses ITK to SYK in unspecified peripheral T-cell lymphoma
Leukemia (2006)
-
Comparative genomic hybridization analysis of thymic neuroendocrine tumors
Modern Pathology (2005)