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Epigenetic change in IGF2R is associated with fetal overgrowth after sheep embryo culture

Abstract

Manipulation or non-physiological embryo culture environments can lead to defective fetal programming in livestock. Our demonstration of reduced fetal methylation and expression of ovine IGF2R suggests pre-implantation embryo procedures may be vulnerable to epigenetic alterations in imprinted genes. This highlights the potential benefits of epigenetic diagnostic screening in developing embryo procedures.

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Figure 1: Validation of relative RT–PCR assay and ovine IGF2R/DMR2 identification.

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Acknowledgements

We thank P. Lobel for the IGF2R antibody and purified IGF2R. This work was supported by MAFF and SOAEFD.

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Correspondence to Lorraine E. Young.

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Young, L., Fernandes, K., McEvoy, T. et al. Epigenetic change in IGF2R is associated with fetal overgrowth after sheep embryo culture. Nat Genet 27, 153–154 (2001). https://doi.org/10.1038/84769

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