Abstract
Manipulation or non-physiological embryo culture environments can lead to defective fetal programming in livestock. Our demonstration of reduced fetal methylation and expression of ovine IGF2R suggests pre-implantation embryo procedures may be vulnerable to epigenetic alterations in imprinted genes. This highlights the potential benefits of epigenetic diagnostic screening in developing embryo procedures.
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Acknowledgements
We thank P. Lobel for the IGF2R antibody and purified IGF2R. This work was supported by MAFF and SOAEFD.
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Young, L., Fernandes, K., McEvoy, T. et al. Epigenetic change in IGF2R is associated with fetal overgrowth after sheep embryo culture. Nat Genet 27, 153–154 (2001). https://doi.org/10.1038/84769
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DOI: https://doi.org/10.1038/84769
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