Abstract
CASPERSSON et al.1,2 have reported the staining of mitotic chromosomes in various organisms using fluorescent acridine derivatives. According to Zech3, the distal portion of the human Y chromosome fluoresces brightly when stained with quinacrine mustard. Vosa4 has observed similar fluorescence using quinacrine dihydro-chloride, which is more easily obtainable. We therefore investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome.
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References
Caspersson, T., Farber, S., Foley, G. E., Kudynowski, J., Modest, E. J., Simonsson, E., Wagh, U., and Zech, L., Exp. Cell Res., 49, 219 (1968).
Caspersson, T., Zech, L., Modest, E. J., Foley, G. E., Wagh, U., and Simonsson, E., Exp. Cell Res., 58, 141 (1969).
Zech, L., Exp. Cell Res., 58, 463 (1969).
Vosa, C. G., Chromosoma (in the press).
Barr, M. L., and Bertram, E. G., Nature, 163, 676 (1949).
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PEARSON, P., BOBROW, M. & VOSA, C. Technique for Identifying Y Chromosomes in Human Interphase Nuclei. Nature 226, 78–80 (1970). https://doi.org/10.1038/226078a0
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DOI: https://doi.org/10.1038/226078a0
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