Abstract
Purpose: We previously demonstrated that aneuploidy-free oocytes may be preselected by testing the first and second polar bodies removed from oocytes following their maturation and fertilization. The present paper describes the results of the application of the method in 659 in vitro fertilization cycles from patients of advanced maternal age.
Methods: Using micromanipulation techniques, 3943 oocytes were tested by polar body sampling and fluorescent in situ hybridization analysis using specific probes for chromosomes 13, 18, and 21.
Results: Fluorescent in situ hybridization results were available for 3217 (81.6%) of 3943 oocytes studied, of which 1388 (43.1%) had aneuploidies; 35.7% of the aneuploidies were of first meiotic division origin, and 26.1% of second meiotic division origin. Most errors in the first meiotic division were represented by chromatid malsegregation. The transfer of embryos deriving from 1558 of 1829 aneuploidy-free oocytes in 614 treatment cycles resulted in 131 clinical pregnancies and 88 healthy children born after confirmation of the polar body diagnosis.
Conclusions: Polar body testing of oocytes provides an accurate and reliable approach for prevention of age-related aneuploidies in in vitro fertilization patients of advanced maternal age.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Verlinsky Y, Rechitsky S, Cieslak, J, Ivakhnenko V, Wolf G, Lifchez A, Kaplan B, Moise J, Walle J, White M, Ginsberg N, Strom C, Kuliev A: Preimplantation diagnosis of single gene disorders by two-step oocyte genetic analysis using first and second polar body. Biochem Mol Med 1997;62:182-187
Verlinsky Y, Cieslak J, Ivakhnenko V, Lifchez A, Strom C, Kuliev A: Birth of healthy children after preimplantation diagnosis of common aneuploidies by polar body fluorescent in situ hybridization analysis. Fertil Steril 1996;66(1):126-129
Verlinsky Y, Cieslak J, Ivakhnenko V, Evsikov S, Wolf G, White M, Lifchez A, Kaplan B, Moise J, Valle J, Ginsberg N, Srom C, Kuliev A: Preimplantation diagnosis of common aneuploidies by first-and second-polar body FISH analysis. J Assist Reprod Genet 1998;15:285-289
Verlinsky Y, Cieslak J, Ivakhnenko V, et al.: Prepregnancy genetic testing for age-related aneuploidies by polar body analysis. Genet Test 1998;1(4):231-235
Nicolaides P, Petersen M: Origin and mechanisms of non-disjunction in human autosomal trisomies. Hum Reprod 1998;13(2):313-319
Battaglia DE, Goodwin P, Klein NA, Soules MR: Influence of maternal age on meiotic spindle assembly in oocytes from naturally cycling women. Hum Reprod 1996;11:2217-2222
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Verlinsky, Y., Cieslak, J., Ivakhnenko, V. et al. Prevention of Age-Related Aneuploidies by Polar Body Testing of Oocytes. J Assist Reprod Genet 16, 165–169 (1999). https://doi.org/10.1023/A:1020304621338
Issue Date:
DOI: https://doi.org/10.1023/A:1020304621338