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Formation of homocitrulline during heating of milk

Published online by Cambridge University Press:  01 November 1998

ALI A. M. METWALLI
Affiliation:
Department of Food Science, Wageningen Agricultural University, PO Box 8129, NL-6700 EV Wageningen, The Netherlands Permanent address: Department of Dairying, Faculty of Agriculture, University of El-Minia, El-Minia, Egypt.
WIM L. LAMMERS
Affiliation:
Department of Food Science, Wageningen Agricultural University, PO Box 8129, NL-6700 EV Wageningen, The Netherlands
MARTINUS A. J. S. VAN BOEKEL
Affiliation:
Department of Food Science, Wageningen Agricultural University, PO Box 8129, NL-6700 EV Wageningen, The Netherlands

Abstract

Homocitrulline arises from the reaction between cyanate and the ε-amino group of lysine residues; in milk, cyanate derives from heat-induced urea breakdown. Since homocitrulline levels were unknown in heated milk, its formation was studied in the temperature range 100–150°C. Firstly, an analysis method based on ion-exchange chromatography using an amino acid analyser was developed. Homocitrulline, liberated from milk protein by enzymic hydrolysis, was eluted well separated from other amino acids and could be readily distinguished using this technique. Secondly, homocitrulline levels were determined for various time–temperature combinations in samples of milk, milk to which 10 mm-urea had been added, and milk that was made urea-free. No homocitrulline was formed in urea-free milk, while homocitrulline formation was stimulated by urea addition. Upon prolonged heating, we found extensive subsequent breakdown of homocitrulline. The kinetics of homocitrulline formation was quite complicated, but an approximation was possible as the initial formation reaction could be modelled using zero order reaction kinetics. The apparent activation energy for homocitrulline formation was estimated at ∼90 kJ/mol. In general, the levels of homocitrulline to be expected in heated milk appeared to be quite low: ∼0·3 mm in in-bottle sterilized milk, and <0·01 mm in UHT sterilized milk.

Type
Research Article
Copyright
Proprietors of Journal of Dairy Research 1998

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