IL-9 and its receptor in allergic and nonallergic lung disease: Increased expression in asthma☆,☆☆,★
Section snippets
Subjects
The subjects in this study were recruited from the outpatient clinic at the Montreal Chest Hospital and from Calmette Hospital, Department of Pneumoimmunoallergy, in Lille, France. Hospital ethics committee approval was obtained for this study, and all participating subjects gave informed written consent before the study. A total of 49 subjects were included in this study. Clinical and demographic characteristics of the patients are shown in Table I.
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RESULTS
Hybridization between the labeled cRNA probes and mRNA encoding IL-9 and IL-9R were demonstrated by specific deposits of silver grains in the photographic emulsion overlying the tissue sections. As shown in Fig 1, we demonstrated the expression of IL-9 mRNA in all asthmatic (Fig 2, A and B ), atopic, and nonatopic control subjects and subjects with chronic bronchitis (Fig 2, C ) and sarcoidosis.
DISCUSSION
In this study we investigated the in vivo expression of IL-9 in human bronchial tissue obtained from atopic asthmatic patients compared with atopic and nonatopic control subjects and with subjects with chronic bronchitis and sarcoidosis. With use of in situ hybridization and immunocytochemistry staining techniques, our results provide direct evidence that IL-9 is expressed both at the gene and protein level in human airways. Its expression, however, was found to be up-regulated significantly in
Acknowledgements
We thank Elsa Schotman and Dr Rame A. Taha for their technical assistance and instructive discussions.
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Supported by the Medical Research Council of Canada and by Magainin Pharmaceuticals, Inc.
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Reprint requests: Qutayba A. Hamid, MD, PhD, Meakins-Christie Laboratories, 3626 St Urbain St, Montreal, Quebec, H2X 2P2 Canada.
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