Human caspase 12 has acquired deleterious mutations
Section snippets
Materials and methods
cDNA synthesis and polymerase chain reaction (PCR). Poly(A)+ RNA (0.5 μg) from multiple tissues (Clontech, USA) and 1.5 μg oligo(dT)15 primer (0.5 μg/μl) were denatured for 15 min at 70 °C in 11 μl diethyl pyrocarbonate-treated water (ADDEPC). For cDNA synthesis the following reagents were added: 5 μl 5× AMV buffer (Roche, Switzerland), 2.5 μl dNTP mix 10 mM each (TaKaRa, Japan), 1.5 μl RNasin (40 U/μl) (Roche, Switzerland), 1.5 μl AMV reverse transcriptase (10 U/μl) (Roche, Switzerland), and 2 μl ADDEPC and
Results and discussion
BLAST search of the human genome working draft [12] (http://www.ncbi.nlm.nih.gov/genome/seq/HsBlast.html), which covers 98.5% of the human genome sequence, with the full length sequence of the murine caspase 12 cDNA (GenBank Acc No. NM_009808) [6], revealed a single caspase 12 locus consisting of at least eight putative exons on the chromosome 11q22.3. The gene of caspase 12 is located within a region of genes of the interleukin-1β converting enzyme (ICE) subfamily of caspases (caspases 1, 4,
Acknowledgements
We thank Heidi Rossiter for a critical reading of the manuscript and Aumaid Uthman for the technical advice.
References (18)
- et al.
Human ICE/CED-3 protease nomenclature
Cell
(1996) - et al.
Caspases: killer proteases
Trends Biochem. Sci.
(1997) - et al.
Characterization of seven murine caspase family members
FEBS Lett.
(1997) - et al.
Evidence that caspase-13 is not a human but a bovine gene
Biochem. Biophys. Res. Commun.
(2001) - et al.
Activation of caspase-12, an endoplastic reticulum (ER) resident caspase, through tumor necrosis factor receptor-associated factor 2-dependent mechanism in response to the ER stress
J. Biol. Chem.
(2001) - et al.
Cop a caspase recruitment domain-containing protein and inhibitor of caspase-1 activation processing
J. Biol. Chem.
(2001) - et al.
ICEBERG: a novel inhibitor of interleukin-1beta generation
Cell
(2000) - et al.
Crystal structure of the cysteine protease interleukin-1 beta-converting enzyme: a (p20/p10)2 homodimer
Cell
(1994) - et al.
Caspase activation without death
Cell Death Differ.
(1999)
Cited by (320)
Characterization of caspase gene family in Sebastes schlegelii and their expression profiles under Aeromonas salmonicida and Vibrio anguillarum infection
2024, Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular BiologyInterlacing the relevance of caspase activation in the onset and progression of Alzheimer's disease
2023, Brain Research BulletinCaspase mechanisms in the regulation of inflammation
2022, Molecular Aspects of MedicineMitochondrial calpain-5 truncates caspase-4 during endoplasmic reticulum stress
2022, Biochemical and Biophysical Research Communications
- 1
These authors contribute equally to this paper.