In vitro transcription/translation assay for the screening of hMLH1 and hMSH2 mutations in familial colon cancer☆
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Cited by (62)
Germline characterization of early-aged onset of hereditary non-polyposis colorectal cancer
2001, Journal of PediatricsCitation Excerpt :PCR amplifications were performed by using primers for hMLH1 complementary DNA to amplify the gene into 2 overlapping segments. IVTT was performed, and the product was electrophoresed onto a 12.5% sodium dodecylsulfate–polyacrylamide gel as previously described.19 The gel was fixed and prepared for fluorography with Entensity (New England Nuclear, Beverly, Mass).
AGA technical review on hereditary colorectal cancer and genetic testing
2001, GastroenterologyDNA and RNA analyses in detection of genetic predisposition to cancer
2012, Hereditary Cancer in Clinical PracticeAn American founder mutation in MLH1
2012, International Journal of CancerAn ELISA-based high throughput protein truncation test for inherited breast cancer
2010, Breast Cancer Research
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Supported by National Institutes of Health grant CA46592-06 from the University of Michigan Comprehensive Cancer Center, U.S. Public Health Service grant CA39233 from the Research Service of the Department of Veterans Affairs, and the Johnson Family Fund for Familial Colon Cancer Research.
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Dr. Koi's current affiliation is: Laboratory of Molecular Carcinogenesis, National Institute for Environmental Health Sciences, Research Triangle Park, North Carolina.