Elsevier

Developmental Biology

Volume 105, Issue 2, October 1984, Pages 451-457
Developmental Biology

Full paper
Extracellular matrix mediates epithelial effects on chondrogenesis in vitro

https://doi.org/10.1016/0012-1606(84)90302-6Get rights and content

Abstract

It has been previously observed that single chick embryonic limb mesenchymal cells can differentiate into chondrocytes without cell-cell interactions when cultured in collagen or agarose gels. In the present study, limb ectoderm, but not dermis, inhibits chondrogenesis when placed on such collagen gel cultures. The inhibitory influence can be transmitted extensive distances in the gel, even when the ectoderm is placed on a porous filter. Collagen gels, preconditioned with limb ectoderms, are also inhibitory to chondrogenesis. On the other hand, chondrogenesis is less inhibited by ectoderm when the mesenchymal cells are placed in agarose. These results suggest that the antichondrogenic effect of limb ectoderm is mediated through alterations of the collagenous extracellular matrix and support the idea that the extracellular matrix must be considered as an organized, functional unit capable of regulating cell differentiation.

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    This study was supported by NIH Grants HD05505 and T32 HD07216 to M.S. and EY05191 and AM03564 to T.F.L.

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