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A new rare distamycin a-inducible fragile site, fra(11)(p15.1), found in two acute nonlymphocytic leukemia (ANLL) patients with t(7;11)(p15-p13;p15)

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Summary

Fragile sites were analyzed in normal peripheral lymphocytes from two acute nonlymphocytic leukemia patients with t(7;11)(p15-p13;p15) leukemic cells. To induce expression of fragile sites, cultures were exposed to folate deprivation (M-F10), BrdU, distamycin A, or Hoechst 33258. Fragility at 11p15.1 was induced by distamycin A and Hoechst 33258 but was not seen in M-F10, BrdU, and control cultures. Fra(11)(p15.1) was found neither in healthy Japanese subjects (0 in 845) nor in patients with leukemia or other hematologic disorders without the t(7;11) (0 in 126). From these results, fra(11)(p15.1) can now be calssified as a rare distamycin A-inducible fragile site. Furthermore, this fra(11)(p15.1) coincided with one of the breakpoints of the t(7;11)(p15-p13;p15).

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References

  • Arthur DC, Asseng SM, Bloomfield CD (1985) Acute myelomonocytic leukemia with marrow eosinophilia (M4Eo) and inv(16) (p13q22) in a patient with heritable fragile site 16q22. (8th International Workshop on Human Gene Mapping). Cytogenet Cell Genet 40:572

    Google Scholar 

  • Berger R, Bloomfield CD, Sutherland GR (1985) Report of the committee on chromosome rearrangements in neoplasia and on fragile sites. (8th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 40:490–535

    Google Scholar 

  • Gollin SM, Perrot LJ, Gray BA, Kletzel M (1986) Spontaneous expression of fra(11)(p23) in a patient with Ewing's sarcoma and t(11;22)(q23;q11). Cancer Genet Cytogenet 20:331–339

    Google Scholar 

  • Hori T, Takahashi E, Ishihara T, Minamihisamatsu M, Kaneko Y, Murata M (1988) Distamycin A-iducible fragile sites and cancer-proneness. Cancer Genet Cytogenet (in press)

  • ISCN (1985) An international system for human cytogenetic nomenclature. In: Harnden DG, Klinger HP (eds) Published in collaboration with Cytogenet Cell Genet. Karger, Basel

    Google Scholar 

  • Ishihara T, Minamihisamatsu M, Yokoyama Y (1986) Translocation t(7;11)(p13;p15) in acute nonlymphocytic leukemia. Cancer Genet Cytogenet 19:363–364

    Google Scholar 

  • LeBeau MM (1988) Editoral: Chromosomal fragile sites and cancer-specific breakpoints — a moderating viewpoint. Cancer Genet Cytogenet 31:55–61

    Google Scholar 

  • LeBeau MM, Rowley JD (1984) Heritable fragile sites in cancer. Nature 308:607–608

    Google Scholar 

  • Murata M, Takahashi E, Ishihara T, Minamihisamatsu M, Takagi T, Kaneko Y, Hori T (1987) Heritable fragile sites and cancer: fra(16)(q22) in lymphocytes of an acute nonlymphocytic leukemia patient with inv(16)(p13q22). Cancer Genet Cytogenet 25:81–86

    Google Scholar 

  • Murata M, Takahashi E, Minamihisamatsu M, Ishihara T, Wong P, Bessho M, Hirashima K, Hori T (1988) Heritable rare fragile sites in patients with leukemia and other hematologic disorders. Cancer Genet Cytogenet 31:95–103

    Google Scholar 

  • Puspurs AH, Baker E, Callen DF, Fratini A, Sutherland GR (1988) Translocation breakpoint at 11q13.3 in the t(11;14) in B-cell leukemia is not at the rare fragile site at 11q13.3. Cancer Genet Cytogenet 31:25–30

    Google Scholar 

  • Simmers RN, Sutherland GR (1988) Further localization of ETS1 indicates that the chromosomal rearrangement in Ewing sarcoma does not occur at fra(11)(q23). Hum Genet 78:144–147

    Google Scholar 

  • Simmers RN, Sutherland GR, West A, Richards RI (1987) The fragile sites at 16q22 are not located at the breakpoint of the chromosomal rearrangement in acute myelomonocytic leukemia. Science 236:92–94

    Google Scholar 

  • Sutherland GR (1988) Editorial: Fragile sites and cancer breakpoints — the pessimistic view. Cancer Genet Cytogenet 31:5–7

    Google Scholar 

  • Takahashi E, Hori T, Murata M (1988) Population cytogenetics of rare fragile sites in Japan. Hum Genet 78:121–126

    Google Scholar 

  • Yunis JJ (1983) The chromosomal basis of human neoplasia. Science 221:227–228

    Google Scholar 

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Takahashi, Ei., Kaneko, Y., Ishihara, T. et al. A new rare distamycin a-inducible fragile site, fra(11)(p15.1), found in two acute nonlymphocytic leukemia (ANLL) patients with t(7;11)(p15-p13;p15). Hum Genet 80, 124–126 (1988). https://doi.org/10.1007/BF00702853

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  • DOI: https://doi.org/10.1007/BF00702853

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