Abstract
Total cytoplasmic polyadenylated RNA was isolated from the human lymphocyte cell line Wil2 by oligo(dT)-cellulose chromatography. Tritiated complementary DNA (cDNA) was transcribed from the RNA and used as a probe for in situ hybridization to metaphase chromosomes. The majority of G-negative or lightly staining bands were found to be preferential sites of hybridization.
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Yunis, J.J., Kuo, M.T. & Saunders, G.F. Localization of sequences specifying messenger RNA to light-staining G-bands of human chromosomes. Chromosoma 61, 335–344 (1977). https://doi.org/10.1007/BF00288617
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DOI: https://doi.org/10.1007/BF00288617