Summary
A treatment by BrdU during 24 hrs, followed by a treatment by thymidine, during 30 hrs, induces in the same preparations, many types of chromosome banding R-, Q-, H-bands, and several types of asymmetry.
This indicates that the BrdU is not incorporated only in some parts of the karyotype, but, on the contrary, any where, and then induces different banding according to the site of its incorpotation.
Above all, the type of band is determined by the period of the phase S in which the cell was at the time of the treatment by BrdU. Thus the coexistence of many types of banding may be explained here by the great variability of the cellular cycle of the lymphocytes in culture.
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Bobrow, M., Madan, K., Pearson, P.: Staining of some specific regions of human chromosomes, particularly the secondary constriction region of n0 9. Nature (Lond.) New Biol. 238, 122–124 (1972)
Couturier, J., Dutrillaux, B., Lejeune, J.: Etudes des fluorescences spécifiques des bandes R et des bandes Q des chromosomes humains. C.R. Acad. Sci. (Paris) 276, 339–342 (1973)
Dutrillaux, B.: Nouveau système de marquage chromosomique: les bandes T. Chromosoma (Berl.) 41, 395–402 (1973)
Dutrillaux, B.: Sur la nature et l'origine des chromosomes humains. Monographie des Annales de Génétique. Paris: l'Expansion Scientifique 1975
Dutrillaux, B., Aurias, A., Fosse, A. M.: Différenciation des mécanismes induisant la segmentation et l'asymétrie des chromatides, aprés traitement par le BudR (5 bromodéoxyuridine). Exp. Cell Res. (sous presse)
Dutrillaux, B., Fosse, A. M.: Sur la segmentation chromosomique induite par le BudR (5 bromodéoxyuridine). Ann. Génét. 17, 207–211 (1974)
Dutrillaux, B., Fosse, A. M., Prieur, M., Lejeune, J.: Analyse des échanges de chromatides dans les cellules somatiques humaines. Chromosoma (Berl.) 48, 327–340 (1974)
Dutrillaux, B., Laurent, C., Couturier, J., Lejeune, J.: Coloration par l'acridine orange, de chromosomes préalablement traités par le 5 bromodéoxyuridine (BudR). C.R. Acad. Sci. (Paris) 276, 3179–3181 (1973)
Dutrillaux, B., Lejeune, J.: Sur une nouvelle technique d'analyse du caryotype humain. C.R. Acad. Sci. (Paris) 272, 2638–2640 (1971)
Gagné, R., Laberge, C.: Specific cytological recogniting of the heterochromatic segment of number 9 chromosome in man. Exp. Cell Res. 73, 239–242 (1972)
Ikushima, T., Wolff, S.: Sister chromatid exchanges induced by light flashes to 5-bromodeoxyuridine an 5-iododeoxyuridine substituted chinese hamster chromosomes. Exp. Cell Res. 87, 15–19 (1974)
Latt, S. A.: Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes. Proc. nat. Acad. Sci. (Wash.) 70, 3395–3399 (1973)
Palmer, C. G.: 5-bromodeoxyuridine induced constrictions in human chromosomes. Canad. J. Genet. Cytol. 12, 816–830 (1970)
Zakharov, A. F., Egolina, N. A.: Differential spiralization along mammalian mitotic chromosomes. I. BudR revealed differentiation in chinese hamster chromosomes. Chromosoma (Berl.) 38, 341–366 (1972)
Zakharov, A., Seleznev, J. V., Benjusch, V. A., Baranovskaya, L. I., Demintseva, V. S.: Differentiation along human chromosomes in relation to their identification. Excerpta med., Intern. Congress series 233, 193 (1971)
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Collaboration technique: Anne Marie Fosse
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Dutrillaux, B. Obtention simultanée de plusieurs marquages chromosomiques sur les mêmes préparations, après traitement par le BrdU. Hum Genet 30, 297–306 (1975). https://doi.org/10.1007/BF00275142
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DOI: https://doi.org/10.1007/BF00275142